Biomimetic electrospun nanofibrous scaffold for tissue engineering: preparation, optimization by design of experiments (DOE), in-vitro and in-vivo characterization

IF 4.3 3区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Frontiers in Bioengineering and Biotechnology Pub Date : 2023-10-31 DOI:10.3389/fbioe.2023.1288539

Shabnam Anjum, Ting Li, Dilip Kumar Arya, Daoud Ali, Saud Alarifi, Wang Yulin, Zhang Hengtong, P. S. Rajinikanth, Qiang Ao

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引用次数: 0

Abstract

Electrospinning is a versatile method for fabrication of précised nanofibrous materials for various biomedical application including tissue engineering and drug delivery. This research is aimed to fabricate the PVP/PVA nanofiber scaffold by novel electrospinning technique and to investigate the impact of process parameters (flow rate, voltage and distance) and polymer concentration/solvent combinations influence on properties of electrospun nanofibers. The in-vitro and in-vivo degradation studies were performed to evaluate the potential of electrospun PVP/PVA as a tissue engineering scaffold. The solvents used for electrospinning of PVP/PVA nanofibers were ethanol and 90% acetic acid, optimized with central composite design via Design Expert software. NF-2 and NF-35 were selected as optimised nanofiber formulation in acetic acid and ethanol, and their characterization showed diameter of 150–400 nm, tensile strength of 18.3 and 13.1 MPa, respectively. XRD data revealed the amorphous nature, and exhibited hydrophilicity (contact angles: 67.89° and 58.31° for NF-2 and NF-35). Swelling and in-vitro degradability studies displayed extended water retention as well as delayed degradation. FTIR analysis confirmed solvent-independent interactions. Additionally, hemolysis and in-vitro cytotoxicity studies revealed the non-toxic nature of fabricated scaffolds on RBCs and L929 fibroblast cells. Subcutaneous rat implantation assessed tissue response, month-long biodegradation, and biocompatibility through histological analysis of surrounding tissue. Due to its excellent biocompatibility, this porous PVP/PVA nanofiber has great potential for biomedical applications.

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组织工程用仿生静电纺纳米纤维支架:制备、实验设计优化(DOE)、体外和体内表征

静电纺丝是一种多用途的制备纳米纤维材料的方法，可用于多种生物医学应用，包括组织工程和药物输送。本研究旨在利用新型静电纺丝技术制备PVP/PVA纳米纤维支架，并研究工艺参数(流速、电压和距离)和聚合物浓度/溶剂组合对静电纺丝纳米纤维性能的影响。通过体外和体内降解研究来评估静电纺丝PVP/PVA作为组织工程支架的潜力。静电纺丝PVP/PVA纳米纤维的溶剂为乙醇和90%乙酸，并通过design Expert软件进行中心复合设计优化。以NF-2和NF-35为优化配方，在醋酸和乙醇条件下制备的纳米纤维直径为150 ~ 400 nm，抗拉强度分别为18.3和13.1 MPa。XRD数据显示，NF-2和NF-35具有亲水性(接触角分别为67.89°和58.31°)。溶胀和体外降解性研究显示延长水潴留和延迟降解。FTIR分析证实了与溶剂无关的相互作用。此外，溶血和体外细胞毒性研究表明，制备的支架对红细胞和L929成纤维细胞无毒。大鼠皮下植入通过周围组织的组织学分析来评估组织反应、一个月的生物降解和生物相容性。由于其优异的生物相容性，这种多孔PVP/PVA纳米纤维在生物医学上具有很大的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Bioengineering and Biotechnology Chemical Engineering-Bioengineering

CiteScore

8.30

自引率

5.30%

发文量

2270

审稿时长

12 weeks

期刊介绍： The translation of new discoveries in medicine to clinical routine has never been easy. During the second half of the last century, thanks to the progress in chemistry, biochemistry and pharmacology, we have seen the development and the application of a large number of drugs and devices aimed at the treatment of symptoms, blocking unwanted pathways and, in the case of infectious diseases, fighting the micro-organisms responsible. However, we are facing, today, a dramatic change in the therapeutic approach to pathologies and diseases. Indeed, the challenge of the present and the next decade is to fully restore the physiological status of the diseased organism and to completely regenerate tissue and organs when they are so seriously affected that treatments cannot be limited to the repression of symptoms or to the repair of damage. This is being made possible thanks to the major developments made in basic cell and molecular biology, including stem cell science, growth factor delivery, gene isolation and transfection, the advances in bioengineering and nanotechnology, including development of new biomaterials, biofabrication technologies and use of bioreactors, and the big improvements in diagnostic tools and imaging of cells, tissues and organs. In today`s world, an enhancement of communication between multidisciplinary experts, together with the promotion of joint projects and close collaborations among scientists, engineers, industry people, regulatory agencies and physicians are absolute requirements for the success of any attempt to develop and clinically apply a new biological therapy or an innovative device involving the collective use of biomaterials, cells and/or bioactive molecules. “Frontiers in Bioengineering and Biotechnology” aspires to be a forum for all people involved in the process by bridging the gap too often existing between a discovery in the basic sciences and its clinical application.