Phytochemical Screening, GC-MS Analysis and Total Phenolic Content Determination of Bioactive Compound of Propolis

Q4 Pharmacology, Toxicology and Pharmaceutics

International Journal of Drug Delivery Technology Pub Date : 2023-09-25 DOI:10.25258/ijddt.13.3.18

Meenu ., Tarun Virmani, Lubhan Singh, Rahul Kaushik

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Abstract

India is a hub for a large variety of natural products that have proven their therapeutic efficacy in various ailments and disorders. Despite it, a lot of natural products are yet to be explored, and Indian propolis is one of them. Indian propolis is a waxy natural product obtained from beehives. In this research, phytochemical constituents present in Indian propolis were qualitatively analyzed using phytochemical screening, gas chromatography-mass spectroscopy (GC-MS), and quantitatively using total phenolic content. Phytochemical screening results revealed the presence of total phenolic content, saponins, glycosides, alkaloids, terpenoids, proteins, steroids, flavonoids, oils & resins supported by GC-MS results. The GC-MS analysis revealed the presence of 17 different constituents having higher peak areas. Amongst these 17 bioactive compounds, oxirane, phenyl ethyl alcohol, caffeine, chrysene, riboflavin and 2-aminophenol were found to have higher peak areas of 5.78, 4.58, 4.46, 3.11, 2.65 and 2.56%, respectively. The results of total phenolic contents revealed the presence of 23 different constituents in different concentrations amongst ellagic acid, kaempferol, epicatechin, galangin, and quercetin were present in rich amounts 538.73, 402.34, 234.85, 121.95 and 95.3 μg/kg, respectively. These constituents are well known for their therapeutic efficacy, which is an indication for the exploration of the therapeutic efficacy of Indian propolis in the future.

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蜂胶的植物化学筛选、GC-MS分析及总酚含量测定

印度是各种天然产品的中心，这些产品已被证明对各种疾病和失调具有治疗功效。尽管如此，仍有许多天然产品有待开发，印度蜂胶就是其中之一。印度蜂胶是从蜂箱中提取的一种蜡状天然产品。本研究采用植物化学筛选、气相色谱-质谱联用(GC-MS)技术对印度蜂胶中的植物化学成分进行了定性分析，并对总酚含量进行了定量分析。植物化学筛选结果显示，总酚含量、皂苷、苷类、生物碱、萜类、蛋白质、类固醇、黄酮类、油脂等;GC-MS结果支持的树脂。GC-MS分析显示有17种不同的成分具有较高的峰面积。其中，氧环烷、苯乙醇、咖啡因、蒽、核黄素和2-氨基酚的峰面积分别为5.78、4.58、4.46、3.11、2.65和2.56%。总酚含量测定结果显示，花鞣花酸、山奈酚、表儿茶素、高良姜素和槲皮素的含量分别为538.73、402.34、234.85、121.95和95.3 μg/kg。这些成分的治疗功效是众所周知的，这是未来探索印度蜂胶治疗功效的一个指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Drug Delivery Technology Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.70

自引率

0.00%

发文量

期刊介绍： International Journal of Drug Delivery Technology (IJDDT) provides the forum for reporting innovations, production methods, technologies, initiatives and the application of scientific knowledge to the aspects of pharmaceutics, including controlled drug release systems, drug targeting etc. in the form of expert forums, reviews, full research papers, and short communications.