Phytochemical studies of Premna schimperi, and antioxidant activities of the extract and an isolated compound

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-09-25 DOI:10.4314/bcse.v37i6.14

Mohammedamin Nasir Isa, Wendimagegn Mammo, Estifanos Ele Yaya

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Abstract

ABSTRACT. In this study, the attempts were made to isolate secondary metabolites from the solvent extract of the dried leaves of Premna schimperi (P. schimperi). Thus, six compounds, namely, ent-8b,12-epidioxy-12b-hydroxylabda-9(11),13-dien-15-oic acid-g-lactone (1), 3,5,5'-trihydroxy-6,7,3',4'-tetramethoxyflavone (2) and 3,5,7,5'-tetrahydroxy-6,3',4'-trimethoxyflavone (3), p-hydroxycinnamic acid (4), luteolin (5) and 1-nonacosanol (6) were isolated and characterized by NMR, UV-Vis, MS and FTIR spectroscopic techniques. All the compounds; except luteolin (5) were reported for the first time from the leaves of P. schimperi. In addition, the GC-MS analysis of the essential oil of the plant leaves revealed 47 compounds, of which a-curcumene (16.38%), caryophyllene (10.73%), eugenol (6.85%), b-sesquiphellandrene (4.65%), g-gurjunene (4.55%), terpinen-4-ol (2.9%), g-eudesmol (4.43%), linalool (2.2%) and caryophyllene oxide (2.67%) were the major compounds. Furthermore, assessment of the antioxidant activities of the MeOH extract and compound 1 using the DPPH assay revealed 95.3% and 62.2% DPPH inhibition, respectively, at concentration of 100 μg/mL. KEY WORDS: P. schimperi, GC-MS, DPPH radical scavenging assay, Antioxidant activity, Acetylation Bull. Chem. Soc. Ethiop. 2023, 37(6), 1471-1486.DOI: https://dx.doi.org/10.4314/bcse.v37i6.14

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水芹的植物化学研究及其提取物和分离化合物的抗氧化活性

摘要在本研究中，试图从水芹(P. schimperi)干叶的溶剂提取物中分离出次生代谢物。分离得到6个化合物，分别为-8b、12-附二氧基-12b-羟基-9(11)、13-二烯-15-酸-g-内酯(1)、3,5,5,5 '-三羟基-6,7,3'，4'-四甲氧基黄酮(2)、3,5,7,5'-四羟基-6,3'，4'-三甲氧基黄酮(3)、对羟基肉桂酸(4)、木犀草素(5)和1-壬烷醇(6)，并通过NMR、UV-Vis、MS和FTIR等光谱技术进行了表征。所有的化合物;除木犀草素(5)外，均为首次报道。此外，通过GC-MS分析，共鉴定出47种化合物，其中a-姜黄烯(16.38%)、石竹烯(10.73%)、丁香酚(6.85%)、b-倍半黄烯(4.65%)、g-古芦烯(4.55%)、松油烯-4-醇(2.9%)、g-木犀草酚(4.43%)、芳樟醇(2.2%)和石竹烯氧化物(2.67%)为主要化合物。此外，利用DPPH法评估MeOH提取物和化合物1的抗氧化活性，在浓度为100 μg/mL时，DPPH抑制率分别为95.3%和62.2%。【关键词】香茅，GC-MS, DPPH自由基清除试验，抗氧化活性，乙酰化;公牛。化学。Soc。中国生物医学工程学报，2016,37(6)，1471-1486。DOI: https://dx.doi.org/10.4314/bcse.v37i6.14& x0D;

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.