Immunoquantitation of cytochrome b5 and methylcholanthrene-induced cytochromes P-450.

Federation proceedings Pub Date : 1987-06-01

T K Shires, P A Krieter, L K Shawver, S L Seidel

引用次数: 0

Abstract

The enzyme-linked immunosorbent assay (ELISA) has been investigated for its ability to quantitate hydrophobic proteins like cytochromes b5 and P-450 at the subnanogram level. Issues encountered that have broad significance not only for ELISA, but for other qualitative and quantitative immunoassays as well, include the effects of detergent, the discriminatory capacity of ELISA, and the method for determining an assay's selectivity.

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细胞色素b5和甲基胆碱诱导的细胞色素P-450的免疫定量。

酶联免疫吸附试验(ELISA)在亚纳克水平上定量细胞色素b5和P-450等疏水蛋白的能力已被研究。遇到的问题不仅对ELISA有广泛的意义，而且对其他定性和定量免疫测定也有广泛的意义，包括洗涤剂的影响，ELISA的区分能力，以及确定测定选择性的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Federation proceedings

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