Gamma-cyclodextrin functionalized silver nanoparticle-based colorimetric assay for the determination of metformin in pharmaceutical samples

IF 2.3 4区 化学 Q2 Agricultural and Biological Sciences
Pervin Işik Bilgili, Saliha Esin Çelik, Reşat Apak
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引用次数: 0

Abstract

Metformin hydrochloride (MET) (N,N-dimethylbiguanide) is an antidiabetic drug and widely used for treating worldwide disease, Type 2 diabetes. MET shows significant benefits for human health such as reducing high blood sugar and cholesterol/triglyceride levels, treating for polycystic ovary syndrome and having anticancer and antiaging effects. In this study, a facile colorimetric determination of metformin in drug preparations was carried out by environmentally friendly gamma-cyclodextrin (γ-CD) stabilized silver nanoparticles (CD-AgNPs). Gamma-cyclodextrin was used for the formation of AgNPs as both the metal salt − reducing and nanoparticle − stabilizing agent. As a visual optical probe, well-dispersed spherical yellow-colored CD-AgNPs (at an average particle size of 10 ± 2 nm) showing maximal surface plasmon resonance absorption at 405 nm was synthesized by one-step method. In the presence of metformin, dispersed AgNPs get aggregated, and a controlled destabilization of CD-AgNPs and core-centered growth of Ag(0) atoms (20 ± 2 nm) were observed a result of host-guest interaction between CD-AgNPs and metformin. Non-covalent hydrogen bonding between the hydroxyl groups of CD-AgNPs (in the outer cavity) and the guanidine groups of MET enables the CDs to form a host-guest inclusion complexation followed by aggregation leading to color change from yellow to orange and an increase of the absorbance at 550 nm. Inclusion complex formation was characterized by using FTIR, UV-Vis spectroscopy, DSC and STEM techniques. Under optimized conditions, the absorbance ratio at A550 nm/A405 nm was linearly correlated to the concentration of metformin in the range of 0.2–1.25 µM. The proposed assay showed high sensitivity with 42 nM detection limit. The method was selective toward possible interferents (potassium, carbonate, sulfate, chloride and sodium ions, urea, glucose) and also successfully applied to conventional drug samples including MET. The results obtained by proposed, reference and HPLC assays were analyzed statistically using the two-way ANOVA test to demonstrate their agreement with each other at 95% confidence level (P = 0.05, Fexp = 0.068, Fcrit(table) = 6.943, Fexp < Fcrit(table)). MET contents (mg) of drug samples in tablet form were determined with high accuracy (REC% 97.3-100.7) and reproducibility (RSD% 1.49–2.78). It is noteworthy that the developed CD-AgNP − based colorimetric method seems to be of higher or comparable sensitivity compared to other nanoparticle − based optical assays.

Abstract Image

基于γ -环糊精功能化银纳米粒子的比色法测定药品样品中的二甲双胍
盐酸二甲双胍(Metformin hydrochloride, MET) (N,N-二甲双胍)是一种抗糖尿病药物,广泛用于治疗2型糖尿病。MET对人体健康有显著的益处,如降低高血糖和胆固醇/甘油三酯水平,治疗多囊卵巢综合征,具有抗癌和抗衰老作用。本研究采用环境友好型γ-环糊精(γ-CD)稳定银纳米粒子(CD-AgNPs)快速比色法测定药物制剂中的二甲双胍。采用-环糊精作为金属盐还原剂和纳米粒子稳定剂制备AgNPs。采用一步法合成了分散良好的球形CD-AgNPs(平均粒径为10±2 nm),在405 nm处表面等离子体共振吸收最大。在二甲双胍的作用下,分散的AgNPs聚集在一起,CD-AgNPs和二甲双胍的主客体相互作用使Ag(0)原子生长(20±2 nm)。CD-AgNPs的羟基(在外腔中)与MET的胍基之间的非共价氢键使CDs形成主-客体包合,然后聚集,导致颜色从黄色变为橙色,并在550 nm处吸光度增加。采用红外光谱(FTIR)、紫外可见光谱(UV-Vis)、DSC和STEM技术对包合物的形成进行了表征。在优化条件下,A550 nm/A405 nm处的吸光度与二甲双胍浓度在0.2 ~ 1.25µM范围内呈线性相关。该方法灵敏度高,检测限为42 nM。该方法对可能的干扰物(钾、碳酸盐、硫酸盐、氯离子和钠离子、尿素、葡萄糖)具有选择性,也成功地应用于包括MET在内的常规药物样品。建议法、参比法和高效液相色谱法所得结果采用双因素方差分析进行统计分析,在95%的置信水平下,结果一致(P = 0.05, Fexp = 0.068, Fcrit(table) = 6.943, Fexp <Fcrit(表))。测定片剂样品MET含量(mg)的准确度(REC% 97.3 ~ 100.7)和重复性(RSD% 1.49 ~ 2.78)高。值得注意的是,与其他基于纳米颗粒的光学分析相比,开发的基于CD-AgNP -的比色方法似乎具有更高或相当的灵敏度。
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来源期刊
CiteScore
3.30
自引率
8.70%
发文量
0
审稿时长
3-8 weeks
期刊介绍: The Journal of Inclusion Phenomena and Macrocyclic Chemistry is the premier interdisciplinary publication reporting on original research into all aspects of host-guest systems. Examples of specific areas of interest are: the preparation and characterization of new hosts and new host-guest systems, especially those involving macrocyclic ligands; crystallographic, spectroscopic, thermodynamic and theoretical studies; applications in chromatography and inclusion polymerization; enzyme modelling; molecular recognition and catalysis by inclusion compounds; intercalates in biological and non-biological systems, cyclodextrin complexes and their applications in the agriculture, flavoring, food and pharmaceutical industries; synthesis, characterization and applications of zeolites. The journal publishes primarily reports of original research and preliminary communications, provided the latter represent a significant advance in the understanding of inclusion science. Critical reviews dealing with recent advances in the field are a periodic feature of the journal.
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