Effects of Bacillus pumilus on growth performance, serum indexes and muscle development-related gene expression of weanling Jintang black goat

IF 3 4区医学 Q2 MICROBIOLOGY

Beneficial microbes Pub Date : 2023-10-31 DOI:10.1163/18762891-20220093

N. Zhang, J. Ding, J. Li, L. Wang, Y. Wei

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引用次数: 0

Abstract

Abstract Antibiotic misuse has been a severe problem in animal husbandry. It is meaningful to replace antibiotics with Bacillus , as feed additives are indeed a research hotspot. Bacillus pumilus plays a certain role in promoting the growth performance and immunological indicators of animals. There are few reports about the function of goat-derived B. pumilus in animals until now. This study aimed to investigate the effects of B. pumilus fsznc-09 on growth performance and immune function of Jintang black goats. B. pumilus -treated group was fed with 1 ml freeze-dried agent of B. pumilus fsznc-09 at a concentration of 10 9 cfu/ml every 2 days. The growth performance, serum biochemical indexes, the expressions of muscle development and metabolism related genes of Jintang black goats were measured after 30 days. The results showed that the average daily gain and average daily feed intake were significantly increased, and feed conversion ratio was significantly decreased. The activities of total superoxide dismutase, alkaline phosphatase, immunoglobulin G and interferon-γ in serum of goats were significantly increased. However, the activity of malondialdehyde in serum was significantly decreased. The diameters and areas in longissimus dorsi fibre and gluteus fibre of goats were significantly decreased, while the densities in gluteus fibre of goats were significantly increased. The expressions of FAS , LPL , PPAR -γ, CAT , MYOD 1, MYOG , MYF 5 and MyHC I in longissimus dorsi and gluteus of goats were significantly improved. The expressions of 1, SREBP -1, MyHC IIb and MyHC IIx in longissimus dorsi and gluteus of goats were significantly increased. The expressions of FN 1 in longissimus dorsi and MyHC IIa in gluteus of goats were significantly decreased. In conclusion, B. pumilus fsznc-09 can effectively improve the growth performance, immunological indicators and the expressions of muscle development and metabolism related genes of Jintang black goat.

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短小芽孢杆菌对断奶金堂黑山羊生长性能、血清指标及肌肉发育相关基因表达的影响

摘要在畜牧业中，抗生素滥用一直是一个严重的问题。用芽孢杆菌代替抗生素是有意义的，饲料添加剂确实是一个研究热点。短小芽孢杆菌对动物的生长性能和免疫指标有一定的促进作用。目前关于山羊源性矮分枝杆菌在动物体内功能的报道还很少。本试验旨在研究矮螺旋菌fsznc-09对金堂黑山羊生长性能和免疫功能的影响。短柄双歧杆菌处理组每2 d饲喂浓度为10 9 cfu/ml的短柄双歧杆菌fszrc -09冻干剂1 ml。饲喂30 d后，测定金堂黑山羊的生长性能、血清生化指标、肌肉发育及代谢相关基因的表达。结果表明:平均日增重和平均日采食量显著提高，饲料系数显著降低。山羊血清中总超氧化物歧化酶、碱性磷酸酶、免疫球蛋白G和干扰素γ活性显著升高。血清丙二醛活性明显降低。山羊背最长肌纤维和臀肌纤维直径和面积显著降低，臀肌纤维密度显著增加。FAS、LPL、PPAR -γ、CAT、MYOD 1、MYOG、myf5和MyHC I在山羊背最长肌和臀肌中的表达均显著提高。1、SREBP -1、MyHC IIb和MyHC IIx在山羊背最长肌和臀肌中的表达显著升高。山羊背最长肌FN - 1和臀肌MyHC - IIa的表达显著降低。综上所述，矮分枝杆菌fsznc-09能有效提高金塘黑山羊的生长性能、免疫指标及肌肉发育代谢相关基因的表达。

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来源期刊

Beneficial microbes MICROBIOLOGY-NUTRITION & DIETETICS

CiteScore

7.90

自引率

1.90%

发文量

审稿时长

>12 weeks

期刊介绍： Beneficial Microbes is a peer-reviewed scientific journal with a specific area of focus: the promotion of the science of microbes beneficial to the health and wellbeing of man and animal. The journal contains original research papers and critical reviews in all areas dealing with beneficial microbes in both the small and large intestine, together with opinions, a calendar of forthcoming beneficial microbes-related events and book reviews. The journal takes a multidisciplinary approach and focuses on a broad spectrum of issues, including safety aspects of pro- & prebiotics, regulatory aspects, mechanisms of action, health benefits for the host, optimal production processes, screening methods, (meta)genomics, proteomics and metabolomics, host and bacterial physiology, application, and role in health and disease in man and animal. Beneficial Microbes is intended to serve the needs of researchers and professionals from the scientific community and industry, as well as those of policy makers and regulators. The journal will have five major sections: * Food, nutrition and health * Animal nutrition * Processing and application * Regulatory & safety aspects * Medical & health applications In these sections, topics dealt with by Beneficial Microbes include: * Worldwide safety and regulatory issues * Human and animal nutrition and health effects * Latest discoveries in mechanistic studies and screening methods to unravel mode of action * Host physiology related to allergy, inflammation, obesity, etc. * Trends in application of (meta)genomics, proteomics and metabolomics * New developments in how processing optimizes pro- & prebiotics for application * Bacterial physiology related to health benefits