SPİRULİNA PLATENSİS EKSTRESİNİN ANTİ-BÜYÜME, ANTİOKSİDAN VE KARACİĞER KORUYUCU ÖZELLİKLERİ

Abstract

Spirulina platensis (S. platensis), a filamentous cyanobacterium often referred to as a blue-green alga, is recognized for its antioxidant, immunomodulatory, and anti-inflammatory properties. This study aimed to examine the growth-suppressing effects of S. platensis ethanolic extract on PANC-1 and MIA PaCa-2 human pancreatic cancer cells. Additionally, the in vivo hepatoprotective and antioxidant properties of S. platensis were explored. The ethanolic extract of S. platensis was lyophilized and dissolved in DMSO. Subsequently, PANC-1 and MIA PaCa-2 cell lines were exposed to concentrations ranging from 0.1-1000 µg/ml. Cell viability was assessed using sulforhodamine B viability assay. For in vivo evaluation of hepatoprotective and antioxidant properties, S. platensis was administered by gavage at a dose of 50 mg/kg/day over a four-week period. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured in the heart and liver of rats. The control group that received S. platensis showed a significant elevation in the activity of SOD and GSH-Px enzymes in the heart and liver tissues. There was a significant reduction in the ALT and AST enzyme levels. The extract notably hindered the growth of both the examined cell lines. Future research can focus on studying the effects of S. platensis extracts in conjunction with various chemotherapeutic agents or its effect on different cancer cell lines to better understand its anti-growth attributes.