EVALUATION OF THE ANTIPROLIFERATIVE EFFECT OF SAFRANAL IN C-4 I CERVICAL CANCER CELL LINE

Doğu Karadeniz Sağlık Bilimleri Dergisi Pub Date : 2023-10-31 DOI:10.59312/ebshealth.1367528

Souandaou ATHOUMANI ALI, Omur KARABULUT-BULAN, Fatma Gül ÖZCAN

{"title":"EVALUATION OF THE ANTIPROLIFERATIVE EFFECT OF SAFRANAL IN C-4 I CERVICAL CANCER CELL LINE","authors":"Souandaou ATHOUMANI ALI, Omur KARABULUT-BULAN, Fatma Gül ÖZCAN","doi":"10.59312/ebshealth.1367528","DOIUrl":null,"url":null,"abstract":"Aim: Safranal is a bioactive compound responsible for the aroma propriety of Crocus sativus. Many studies have shown the antioxidant activity of safranal besides some pharmacological properties, including its anti-inflammatory effect. Our study aimed on identifying the cytotoxic effects of safranal on the C-4 I cell line of cervical cancer.  Methods: The cytotoxic effect of safranal on the C-4 I cell line was determined after incubating the cells for certain durations (2 to 72 hours) and concentrations (25 to 800 µM). After incubation, cell viability and anti-proliferation effect of safranal were determined respectively by Mitochondrial Dehydrogenase and Lactate dehydrogenase enzymes activities assays. Additionally, morphological changes occurring during incubation in cells were examined with inverted and optical microscope using Giemsa staining.  Results: According to the results, compared to Control group, the % viability of treated cells was decreased depending on concentration and the incubation time, and safranal significantly inhibited the growth of C-4 I cells (p","PeriodicalId":474982,"journal":{"name":"Doğu Karadeniz Sağlık Bilimleri Dergisi","volume":"294 3","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Doğu Karadeniz Sağlık Bilimleri Dergisi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.59312/ebshealth.1367528","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Aim: Safranal is a bioactive compound responsible for the aroma propriety of Crocus sativus. Many studies have shown the antioxidant activity of safranal besides some pharmacological properties, including its anti-inflammatory effect. Our study aimed on identifying the cytotoxic effects of safranal on the C-4 I cell line of cervical cancer. Methods: The cytotoxic effect of safranal on the C-4 I cell line was determined after incubating the cells for certain durations (2 to 72 hours) and concentrations (25 to 800 µM). After incubation, cell viability and anti-proliferation effect of safranal were determined respectively by Mitochondrial Dehydrogenase and Lactate dehydrogenase enzymes activities assays. Additionally, morphological changes occurring during incubation in cells were examined with inverted and optical microscope using Giemsa staining. Results: According to the results, compared to Control group, the % viability of treated cells was decreased depending on concentration and the incubation time, and safranal significantly inhibited the growth of C-4 I cells (p

查看原文本刊更多论文

safranl对c - 4i宫颈癌细胞系抗增殖作用的评价

目的:藏红花醚是藏红花香气的活性成分。许多研究表明，除具有抗炎等药理作用外，番红花醛还具有抗氧化活性。本研究旨在探讨番红花醛对宫颈癌c - 4i细胞系的细胞毒性作用。& # x0D;方法:在一定的培养时间(2 ~ 72小时)和浓度(25 ~ 800µM)下，测定番红花醛对C-4 I细胞株的细胞毒作用。孵育后，分别采用线粒体脱氢酶和乳酸脱氢酶活性测定法检测番红花醛的细胞活力和抗增殖作用。此外，用倒置光学显微镜和吉姆萨染色法观察细胞在孵育过程中发生的形态学变化。& # x0D;结果:与对照组相比，处理细胞的%存活率随浓度和孵育时间而降低，番红花醛显著抑制C-4 I细胞的生长(p

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Doğu Karadeniz Sağlık Bilimleri Dergisi

自引率

0.00%

发文量