Protective Effect of Chlorella vulgaris and Spirulina platensis against ThioacetamideInduced Hepatorenal Toxicity in Male Rats

Abstract

The present inquiry evaluated the possible pharmacological effects of Chlorella vulgaris (CV) and Spirulina platensis (SP) with special reference to their hepatorenal protective effect as well as their antioxidant activities against thioacetamide (TAA) acute toxicity. Thirty-six male Wister albino rats, weighing from 100 to 120 gm were applied in the present study. Rats were randomly allocated into 6groups each of 6 animals. Group I was maintained as the control healthy group. It was administrated distilled water orally for one month and normal saline at a dose of 1 ml IP. On the last two days of the experiment. The other groups were allocated into 5 groups including A, B, C, D and E. Group A was used as a control intoxicated with thioacetamide at a dose of 300 mg/ kg b.wt. IP for two days with 24 hrs intervals before the end of the experiment. Group B was stated as a standard protected group and it was treated with silymarin at a dose of 100 mg/kg b.wt. orally for one month. Group C was given Chlorella vulgaris orally at a quantity of 400 mg/kg b.wt. daily, for one month. Group D was given Spirulina platensis at a dose of 400 mg /kg b.wt. Orally daily for one month. Group E was given CV &SP at doses of 400 mg /kg b.wt each orally daily for one month. Groups B, C, D, and E were intoxicated with thioacetamide (TAA), at an amount of 300mg/kg b.wt. IP for two days with 24 hrs intervals at the end of the month. Toxicity of rats with thioacetamide substantially elevated the levels of alanine transferase and aspartate aminotransferase, serum urea, creatinine, and uric acid in addition to increased malon-di-aldehyde concentration. However, it significantly decreased total proteins, and total antioxidant capacity concentrations and decreased blood parameters. As well as it induced histopathological alterations in hepatic and renal tissue designs (delete). On the other hand, oral administration of Chlorella vulgaris and Spirulina platensis ameliorated TAA-induced biochemical, pathological, and histopathological changes in hepatic tissues and renal tissues. This study stated that these algae attenuate thioacetamide and protect against hepatorenal toxicity, via their antioxidant properties.