Colloidal gold probes in immunocytochemistry. An optimization of their application in light microscopy by use of silver intensification procedures.

Medical biology Pub Date : 1986-01-01
L Scopsi, L I Larsson
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Abstract

Colloidal gold particles are the markers of choice for ultrastructural localization of antigens. By reducing gold chloride with tannic acid and trisodium citrate, a broad range of narrowly determined particle sizes can be obtained. Such particles can easily be coupled to a number of proteins and the resulting conjugates are conveniently purified on a gel-chromatography column. Their application in light microscopy requires an amplification step with a silver physical developer. Silver-intensified colloidal gold probes can advantageously be used for immunostaining of cryostat, paraffin and plastic sections. Moreover, permeabilized cultured cells and whole-mount preparations can also be stained with gold-silver techniques. Silver intensification does not affect reactivity of a number of tissue antigens, thus permitting double staining combinations with immunoperoxidase or immunofluorescence methods.

免疫细胞化学中的胶体金探针。通过使用银强化程序优化其在光学显微镜中的应用。
胶体金颗粒是抗原超微结构定位的首选标记物。通过用单宁酸和柠檬酸三钠还原氯化金,可以获得范围广泛的窄定粒度。这样的颗粒可以很容易地偶联到许多蛋白质上,并且所得的偶联物可以方便地在凝胶层析柱上纯化。它们在光学显微镜中的应用需要用银物理显影剂进行放大步骤。银强化胶体金探针可有利地用于低温恒温器、石蜡和塑料切片的免疫染色。此外,渗透培养细胞和全载制剂也可以用金银技术染色。银强化不影响许多组织抗原的反应性,因此允许与免疫过氧化物酶或免疫荧光方法联合进行双重染色。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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