The Effects of Nano Magnetic Graphene Oxide on In Vivo Maturation of Oocyte

Q3 Engineering
Mitra Rahimi, Tahereh Foroutan, Fatemeh Eini
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引用次数: 0

Abstract

Graphene oxide (GO) and Fe3O4 super paramagnetic material are good candidate for some applications such as drug delivery. It has been shown that combining Fe3O4 with graphene oxide increases the biological efficiency of GO. The use of novel assisted reproductive technologies such as gonadotropins injection has been able to help the fertility of infertile people, but the side effects of these methods and high costs are still problems. The aim of the present study was to investigate the effect of magnetic graphene oxide (MGO) on the in vivo maturation of mouse oocytes. Thirty 6–8-week old female Naval Medical Research Institute (NMRI) mice were treated with intra peritoneal (I.P) injection of MGO mixed with hormones. 12 h after I.P. injection of MGO mixed with PMSG and HCG, the number of metaphase II (MII) oocytes obtained from the left fallopian tubes was counted in each group. Also, immuno-cytochemical staining of glutathione and morphometric analysis of ovaries were studied. The results of this study showed that the simultaneous use of MGO, pregnant mare serum gonadotropin (PMSG), and human chorionic gonadotrophin (HCG) increases the number of MII oocytes and helps to increase maturation of oocytes. It could be concluded that MGO can increase the efficiency of super ovulating hormones due to increase in adsorption of serum hormones and growth factors.
纳米磁性氧化石墨烯对卵母细胞体内成熟的影响
氧化石墨烯(GO)和Fe3O4超顺磁性材料是一些应用的良好候选者,如药物输送。研究表明,氧化石墨烯与Fe3O4结合可提高氧化石墨烯的生物效率。使用新型辅助生殖技术,如注射促性腺激素,已经能够帮助不孕患者的生育能力,但这些方法的副作用和高昂的费用仍然是问题。本研究的目的是探讨磁性氧化石墨烯(MGO)对小鼠卵母细胞体内成熟的影响。本研究采用MGO混合激素腹腔注射治疗30只6 ~ 8周龄海军医学研究所(NMRI)雌性小鼠。注射MGO混合PMSG和HCG 12 h后,计数各组左输卵管中期II (MII)卵母细胞数量。同时对卵巢进行谷胱甘肽免疫细胞化学染色和形态计量学分析。本研究结果表明,同时使用MGO、孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG)可增加MII卵母细胞数量,并有助于促进卵母细胞成熟。由此可见,MGO可通过增加对血清激素和生长因子的吸附来提高超排卵激素的效率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nano Biomedicine and Engineering
Nano Biomedicine and Engineering Engineering-Biomedical Engineering
CiteScore
3.00
自引率
0.00%
发文量
9
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