A review on different types of detectors used in chromatography techniques

Sireesha Badri, Jasmin Rani G, Dharani P, Sreehitha D, Saniya SK, Veeresh C, Chandu K
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引用次数: 0

Abstract

High Performance Thin layer Chromatography (HPTLC) technique is a sophisticated and automated form of the thin-layer chromatography (TLC) with better and advanced separation efficiency and detection limits. The analytical method should be sensitive, specific, fast and accurate to establish the assurance that the equipments used in manufacturing are free of the unwanted impurity, presence of which may alter the safety and efficacy of the drug product. The developed method was based on RP-HPLC leave-taking and quantification of the drug on C-18 column using a mobile phase at flow rate of 1 ml/ min. Quantitation was attained with PDA detector at 200-400 nm based on peak area with linear calibration curves at concentration ranges 5-25 μg/ml for the drug. The method as per ICH guidelines was validated for specificity, linearity, detection limit, quantitation limit, precision, accuracy, robustness, solution stability, and can be effectively used for routine analysis.
色谱技术中不同类型检测器的综述
高效薄层色谱(HPTLC)技术是一种复杂的自动化薄层色谱(TLC)技术,具有更高的分离效率和检出限。分析方法应灵敏、特异、快速和准确,以确保生产设备不含有害杂质,这些杂质的存在可能会改变药品的安全性和有效性。该方法采用反相高效液相色谱法,在C-18柱上进行定量,流动相流速为1 ml/ min。在浓度范围5 ~ 25 μg/ml范围内,根据峰面积,在200 ~ 400 nm范围内采用PDA检测器进行定量。根据ICH指南验证了该方法的特异性、线性度、检出限、定量限、精密度、准确度、鲁棒性、溶液稳定性,可有效用于常规分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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