Prognostic potential of inflammatory markers, oxidative status, thrombocyte indices, and renal biochemical markers in neonatal calf diarrhoea-induced systemic inflammatory response syndrome

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2023-11-01 DOI:10.1016/j.vetimm.2023.110680

Kerim Emre Yanar , Emre Eren , Mustafa Sinan Aktaş , Muhammed Sertaç Eroğlu , Özge Kandemir , Gürsel Aydın

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Abstract

The study aimed to assess the prognostic value of inflammatory markers, indicators of oxidative stress, thrombocyte indices, and renal biochemical markers in neonatal calf diarrhoea (NCD) induced by systemic inflammatory response syndrome (SIRS) upon admission. A prospective, observational, and case-control study was conducted on 56 calves diagnosed with NCD. Mean concentrations of interleukin-6 (IL-6), malondialdehyde (MDA), glutathione (GSH), mean platelet volume (MPV), platelet distribution width (PDW), blood urea nitrogen (BUN), and creatinine (Crea) were measured. Furthermore, the neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) were also calculated for SIRS survivors [SIRS (survivor)] and non-survivors [SIRS (non-survivor)] induced by NCD. A prognostic cut-off value for predicting the prognosis of the SIRS’s induced by NCD was obtained via receiver operating characteristic (ROC) curve analysis. Upon admission, the SIRS (non-survivor) calves had significantly higher (P < .001) average levels of IL-6, MDA, BUN, Crea, MPV, and PDW compared to the SIRS (survivor) calves and significantly lower (P < .001) average levels of GSH. Despite an apparent increase in the NLR and PLR values of calves diagnosed with SIRS, no significant difference was found between the survival and non-survivor SIRS cases. Positive predictive values (PPVs) for survival were determined as 100 %, 100 %, 80 %, 100 %, 80 %, and 80 %, respectively, using cut-off values of IL-6 (≤259.67 ng/L), MDA (≤2.87 nmol/mL), MPV (≤12.5 fL), PDW (≤34.25 %), BUN (≤168.3 mg/dL), and Crea (≤2.11 mg/dL). The determined threshold values are those obtained upon admission to the hospital. Based on the sensitivity, specificity, and PPVs derived from the ROC analysis, it has been concluded that IL-6, MDA, MPV, PDW, BUN, and Crea are the most relevant biomarkers used for predicting the prognosis of NCD-induced SIRS in calves. Furthermore, it is also noteworthy that IL-6 exhibited the highest effectiveness among all biomarkers.

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炎症标志物、氧化状态、血小板指数和肾脏生化标志物在新生儿犊牛腹泻引起的全身炎症反应综合征中的预后潜力

本研究旨在评估入院时炎症标志物、氧化应激指标、血小板指数和肾脏生化指标对新生儿小牛腹泻(NCD)的预后价值。新生儿腹泻由全身炎症反应综合征(SIRS)引起。对56头被诊断为非传染性疾病的小牛进行了一项前瞻性、观察性和病例对照研究。测定白细胞介素-6 (IL-6)、丙二醛(MDA)、谷胱甘肽(GSH)、平均血小板体积(MPV)、血小板分布宽度(PDW)、尿素氮(BUN)、肌酐(Crea)的平均浓度。此外，我们还计算了NCD诱导的SIRS生存者[SIRS (survivor)]和非生存者[SIRS (non-survivor)]的中性粒细胞与淋巴细胞比值(NLR)和血小板与淋巴细胞比值(PLR)。通过受试者工作特征(ROC)曲线分析，获得预测NCD所致SIRS预后的预后临界值。入院时，SIRS(非幸存者)小牛的P <.001) IL-6、MDA、BUN、Crea、MPV和PDW的平均水平与SIRS(幸存者)犊牛相比显著降低(P <.001) GSH的平均水平。尽管被诊断为SIRS的小牛的NLR和PLR值明显增加，但在存活和非存活的SIRS病例之间没有发现显著差异。采用IL-6(≤259.67 ng/L)、MDA(≤2.87 nmol/mL)、MPV(≤12.5 fL)、PDW(≤34.25%)、BUN(≤168.3 mg/dL)和Crea(≤2.11 mg/dL)的临界值，分别确定存活的阳性预测值(ppv)为100%、100%、80%、100%、80%和80%。所确定的阈值是入院时获得的阈值。基于ROC分析得出的敏感性、特异性和ppv，我们得出结论，IL-6、MDA、MPV、PDW、BUN和Crea是预测犊牛非传染性疾病诱导SIRS预后最相关的生物标志物。此外，值得注意的是，IL-6在所有生物标志物中表现出最高的有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.