Zinc lactate alleviates oxidative stress by modulating crosstalk between constitutive androstane receptor signaling pathway and gut microbiota profile in weaned piglets

IF 6.3
Wenjie Tang , Xuan Xiang , Houfu Wang , Wentao Zhou , Liuqin He , Yulong Yin , Tiejun Li
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引用次数: 0

Abstract

This study aimed to determine the regulatory mechanism of dietary zinc lactate (ZL) supplementation on intestinal oxidative stress damage in a paraquat (PQ)-induced piglet model. Twenty-eight piglets (mean body weight 9.51 ± 0.23 kg) weaned at 28 d of age were randomly divided into control, ZL, PQ, and ZL + PQ groups (n = 7 in each group). The ZL-supplemented diet had little effect on growth performance under normal physiological conditions. However, under PQ challenge, ZL supplementation significantly improved average daily gain (P < 0.05) and reduced the frequency of diarrhea. ZL improved intestinal morphology and ultrastructure by significantly increasing the expression level of the jejunal tight junction protein, zonula occludens-1 (ZO-1) (P < 0.05), and intestinal zinc transport and absorption in PQ-induced piglets, which reduced intestinal permeability. ZL supplementation also enhanced the expression of antioxidant and anti-inflammatory factor-related genes and decreased inflammatory cytokine expression and secretion in PQ-induced piglets. Furthermore, ZL treatment significantly inhibited the activation of constitutive androstane receptor (CAR) signaling (P < 0.01) in PQ-induced piglets and altered the structure of the gut microbiota, especially by significantly increasing the abundance of beneficial gut microbes, including UCG_002, Ruminococcus, Rikenellaceae_RC9_gut_group, Christensenellaceae_R_7_group, Treponema, unclassified_Christensenellaceae, and unclassified_Erysipelotrichaceae (P < 0.05). These data reveal that pre-administration of ZL to piglets can suppress intestinal oxidative stress by improving antioxidant and anti-inflammatory capacity and regulating the crosstalk between CAR signaling and gut microbiota.

乳酸锌通过调节断奶仔猪组成型雄甾受体信号通路与肠道菌群之间的串扰来缓解氧化应激
本研究旨在探讨饲粮中添加乳酸锌(ZL)对百草枯(PQ)诱导仔猪肠道氧化应激损伤的调节机制。选取28日龄断奶仔猪28头(平均体重9.51±0.23 kg),随机分为对照组、ZL组、PQ组和ZL + PQ组,每组7头。在正常生理条件下,饲粮中添加zl对生长性能影响不大。然而,在PQ挑战下,添加ZL显著提高了平均日增重(P <0.05),腹泻次数减少。ZL通过显著提高空肠紧密连接蛋白occludenzonula -1 (ZO-1) (P <0.05),降低了pq诱导仔猪肠道锌的运输和吸收,降低了肠道通透性。添加ZL还能提高pq诱导仔猪抗氧化和抗炎因子相关基因的表达,降低炎症细胞因子的表达和分泌。此外,ZL处理显著抑制了组成型雄甾受体(CAR)信号的激活(P <0.01),并改变了肠道微生物群的结构,特别是显著增加了有益肠道微生物的丰度,包括UCG_002、Ruminococcus、Rikenellaceae_RC9_gut_group、christensenellace_r_7_group、密螺旋体、unclassified_Christensenellaceae和unclassified_丹毒杆菌科(P <0.05)。这些数据表明,仔猪预添加ZL可以通过提高抗氧化和抗炎能力以及调节CAR信号与肠道微生物群之间的串扰来抑制肠道氧化应激。
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来源期刊
Animal Nutrition
Animal Nutrition Animal Science and Zoology
CiteScore
9.70
自引率
0.00%
发文量
542
审稿时长
65 days
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.
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