Isolation, characterization, structural elucidation and in vivo hepatoprotective studies of phytoconstituents obtained from the fruits of <i>Cordia obliqua</i> Willd

IF 0.6 4区医学 Q4 PHARMACOLOGY & PHARMACY

Tropical Journal of Pharmaceutical Research Pub Date : 2023-11-06 DOI:10.4314/tjpr.v22i10.15

G. Tharun Babu, S. Sivakrishnan, J.V.C. Sharma

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Abstract

Purpose: To isolate, characterize, and investigate the hepatoprotective effect of phyto-constituents from fruits of Cordia obliqua Wild in paracetamol-induced hepatotoxicity in Wistar rats. Methods: Ethanol and aqueous extracts of C. obliqua fruits were screened for phytochemicals. The extracts were subjected to column chromatography and preparative thin-layer chromatography (TLC) to isolate four novel compounds. Compounds were characterized using infrared spectroscopy (IR), mass spectroscopy (MS), as well as 1H and 13C nuclear magnetic resonance (NMR). The isolated compounds were assessed for acute toxicity, in vivo hepatoprotective and antioxidant potential (dose: 5 mg/kg) in paracetamol-induced (75 mg/kg) hepatotoxicity through oral route in Wistar rats. Results: Phytochemical analysis of ethanol (COE) indicated the presence of fatty acids, anthraquinones, glycosides, saponins, alkaloids, tannins, flavonoids, coumarins, phenolics, triterpenes, and sterols. Compounds A, B, and C were identified from COE. Treatment at 50 mg/kg significantly reduced aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGTP), and total bilirubin (TB), as well as increased total protein and total alkalinity levels in serum compared to the positive control. Liver histo-architecture showed improvements compared to the positive control, indicating hepatic protection. Conclusion: The isolated compounds (A, B and C) from COE exhibit hepatoprotective effects attributed to flavonoids and phenolics with free radical scavenging properties. Further research is needed to identify key mechanisms responsible for hepatoprotective effects.

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Cordia obliqua<果实中植物成分的分离、鉴定、结构鉴定和体内肝保护研究Willd

目的:分离、鉴定和研究斜山茱萸果实植物成分对扑热息痛致Wistar大鼠肝毒性的保护作用。方法:采用醇提液和水提液进行植物化学成分筛选。通过柱层析和制备薄层析分离得到4个新化合物。利用红外光谱(IR)、质谱(MS)、1H和13C核磁共振(NMR)对化合物进行了表征。对经口服扑热息痛致Wistar大鼠肝毒性(75 mg/kg)进行了急性毒性、体内肝保护和抗氧化活性(剂量:5 mg/kg)评价。结果:乙醇(COE)的植物化学分析显示存在脂肪酸、蒽醌类、苷类、皂苷类、生物碱类、单宁类、黄酮类、香豆素类、酚类、三萜和甾醇类。化合物A、B和C从COE中鉴定。与阳性对照组相比，50 mg/kg处理显著降低了天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、碱性磷酸酶(ALP)、γ -谷氨酰转移酶(GGTP)和总胆红素(TB)，提高了血清总蛋白和总碱度水平。与阳性对照组相比，肝脏组织结构有所改善，表明肝脏有保护作用。结论:从黄酮类化合物和酚类化合物中分离得到的化合物A、B和C具有肝脏保护作用，具有清除自由基的作用。需要进一步的研究来确定肝保护作用的关键机制。

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来源期刊

Tropical Journal of Pharmaceutical Research 医学-药学

CiteScore

1.00

自引率

33.30%

发文量

490

审稿时长

4-8 weeks

期刊介绍： We seek to encourage pharmaceutical and allied research of tropical and international relevance and to foster multidisciplinary research and collaboration among scientists, the pharmaceutical industry and the healthcare professionals. We publish articles in pharmaceutical sciences and related disciplines (including biotechnology, cell and molecular biology, drug utilization including adverse drug events, medical and other life sciences, and related engineering fields). Although primarily devoted to original research papers, we welcome reviews on current topics of special interest and relevance.