Palliative Action of Woodfordia fruticosa Leaves Containing Woodfruticosin on Biological Redox Imbalance and Anemia in CFA–Induced Arthritic Rats

IF 0.8 4区医学 Q3 EDUCATION, SCIENTIFIC DISCIPLINES

Indian Journal of Pharmaceutical Education and Research Pub Date : 2023-10-04 DOI:10.5530/ijper.57.4.131

Ashish Singhai, Yusra Ahmad, Umesh Kumar Patil

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Abstract

Abstract: Background: Rheumatoid Arthritis (RA) is a chronic disorder affecting musculoskeletal and autoimmune system with inflammatory characteristics. Anemia is most prevailing, among other extra-articular complications in RA. These complications are analogous to exorbitant inflammation and biological redox imbalance which ultimately affects the quality life and life expectancy of RA patients. Objectives: The study was planned to investigate the palliative action of Woodfordia fruticosa (WF) leaf extracts and isolation from active fraction, on articular/ extra-articular complications in rats with Complete Freund’s Adjuvant (CFA) induced arthritis. Materials and Methods: The action of Petroleum ether (WFP) and Ethanolic (WFE) extracts of WF was assessed in CFA induced adult Lewis rats (180-200gm) arthritic rats. In prophylactic treatment they received WF extracts 100mg/kg p.o. for 28 successive days and therapeutic treatment from the 14th day of CFA injection. Active extract was then fractionated by solvent-solvent extraction and chromatographic techniques. The isolated compound was characterized by TLC and various spectroscopic studies. Results: WFP significantly alleviated the severity of arthritis in animals than WFE in 28 days. Remarkable reduction (p< 0.001) in various complications was observed including paw volume (85.42%), loss in body weight, erythrocyte sedimentation rate, anemia, locomotor activity, and lipid peroxidation of hepatic/ synovial tissues and increment in SOD (U/mg) activities. A compound was isolated and characterized by UV, TLC, FTIR, LCMS and NMR spectroscopic techniques. Conclusion: WFP at 100mg/kg was advantageous in the superintendence of RA intricacies which was also confirmed by the histological and radiological studies. And a tannin compound Woodfruticosin was isolated. Keywords: Rheumatoid arthritis, Chromatography, Isolation, Spectroscopy, Woodfruticosin

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含木果苷的木果叶对慢性关节炎大鼠生物氧化还原失衡和贫血的缓解作用

摘要:背景:类风湿性关节炎(RA)是一种影响肌肉骨骼和自身免疫系统的慢性疾病，具有炎症特征。在RA的其他关节外并发症中，贫血最为普遍。这些并发症类似于过度炎症和生物氧化还原失衡，最终影响RA患者的生活质量和预期寿命。目的:研究木福特叶提取物及其活性部位分离物对完全弗氏佐剂(CFA)诱导的关节炎大鼠关节/关节外并发症的缓解作用。材料与方法:观察石油醚(WFP)和乙醇(WFE)提取物对CFA诱导的成年Lewis大鼠(180 ~ 200gm)关节炎的作用。预防组连续28 d给予白藜芦醇提取物100mg/kg p.o.，治疗组从第14 d开始注射CFA。采用溶剂萃取和色谱技术对活性提取物进行分馏。通过薄层色谱和各种光谱研究对化合物进行了表征。结果:与WFE相比，WFP在28天内明显减轻了动物关节炎的严重程度。脚掌体积(85.42%)、体重下降、红细胞沉降率、贫血、运动活性、肝/滑膜组织脂质过氧化、SOD (U/mg)活性增加等并发症均显著减少(p< 0.001)。通过紫外、薄层色谱、红外光谱、质谱和核磁共振等技术对化合物进行了分离和表征。结论:100mg/kg剂量的WFP对RA复杂性的监测有利，组织学和放射学研究也证实了这一点。分离得到一种单宁化合物木果苷。关键词:类风湿性关节炎;色谱法;分离

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来源期刊

Indian Journal of Pharmaceutical Education and Research 医学-学科教育

CiteScore

1.40

自引率

0.00%

发文量

227

审稿时长

>12 weeks

期刊介绍： The official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967. IJPER, a quarterly publication devoted to publish reviews and research articles in pharmacy and the related disciplines of Pharmaceutical education. It mainly covers the articles of special interest, covering the areas of Pharmaceutical research, teaching and learning, laboratory innovations, education technology, curriculum design, examination reforms, training and other related issues. It encourages debates and discussions on the issues of vital importance to Pharmaceutical education and research. The goal of the journal is to provide the quality publications and publish most important research and review articles in the field of drug development and pharmaceutical education. It is circulated and referred by more than 6000 teachers, 40,000 students and over 1000 professionals working in Pharmaceutical industries, Regulatory departments, hospitals etc.