Biochemical Markers of Liver Damage in Cattle Naturally Infected with Fasciola Hepatica

Aneta Škvareninová, Zuzana Kostecká
{"title":"Biochemical Markers of Liver Damage in Cattle Naturally Infected with Fasciola Hepatica","authors":"Aneta Škvareninová, Zuzana Kostecká","doi":"10.2478/fv-2023-0030","DOIUrl":null,"url":null,"abstract":"Abstract Fascioliasis is a worldwide spread parasitic disease with a high impact on the economy and public health in many countries. Therefore, it is important to apply prevention, effective treatment, and education campaigns, to prevent the disease. This work aimed to determine the biochemical markers of liver damage caused by Fasciola hepatica during 21 days (D0 – D21) post albendazole (ABZ) therapy in the blood sera. This study included 24 cows naturally infected with Fasciola hepatica, randomly divided into a control group (n = 12) and a group treated per os with ABZ (7.5 mg per kg body weight) (n = 12). The activity of hepatic enzymes – aspartate aminotransferase (AST), alanine aminotransferase (ALT) and cholestatic enzymes – gamma-glutamyl transferase (GGT), and alkaline phosphatase (ALP), were measured in both groups. The activity of AST has significantly increased in the course of the experiment (D0 – D21) in both groups (P < 0.001). ALT activity during D0 – D21 was significantly decreased in both groups (P < 0.001). Activity of GGT was not significantly changed in the control group in the course of the experiment (P > 0.05). Contrarily, the activity of GGT in the treated group was significantly decreased (P < 0.01) during D0 – D21. The activity of GGT in the blood sera of the treated group was significantly lower in comparison with the control group in D7 and D21 (P < 0.01). This decreased GGT activity in the blood sera of the treated group may be caused by the effect of albendazole resulting in fasciola elimination and bile flow improvement. The results of ALP determination show no significant changes in ALP activity between groups. This can lead to the conclusion that the most useful marker of cholestasis caused by F. hepatica is the GGT activity and may help to evaluate the ABZ therapy effect in cattle.","PeriodicalId":81274,"journal":{"name":"Folia veterinaria","volume":"374 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Folia veterinaria","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/fv-2023-0030","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Abstract Fascioliasis is a worldwide spread parasitic disease with a high impact on the economy and public health in many countries. Therefore, it is important to apply prevention, effective treatment, and education campaigns, to prevent the disease. This work aimed to determine the biochemical markers of liver damage caused by Fasciola hepatica during 21 days (D0 – D21) post albendazole (ABZ) therapy in the blood sera. This study included 24 cows naturally infected with Fasciola hepatica, randomly divided into a control group (n = 12) and a group treated per os with ABZ (7.5 mg per kg body weight) (n = 12). The activity of hepatic enzymes – aspartate aminotransferase (AST), alanine aminotransferase (ALT) and cholestatic enzymes – gamma-glutamyl transferase (GGT), and alkaline phosphatase (ALP), were measured in both groups. The activity of AST has significantly increased in the course of the experiment (D0 – D21) in both groups (P < 0.001). ALT activity during D0 – D21 was significantly decreased in both groups (P < 0.001). Activity of GGT was not significantly changed in the control group in the course of the experiment (P > 0.05). Contrarily, the activity of GGT in the treated group was significantly decreased (P < 0.01) during D0 – D21. The activity of GGT in the blood sera of the treated group was significantly lower in comparison with the control group in D7 and D21 (P < 0.01). This decreased GGT activity in the blood sera of the treated group may be caused by the effect of albendazole resulting in fasciola elimination and bile flow improvement. The results of ALP determination show no significant changes in ALP activity between groups. This can lead to the conclusion that the most useful marker of cholestasis caused by F. hepatica is the GGT activity and may help to evaluate the ABZ therapy effect in cattle.
牛肝片形吸虫自然感染肝损伤的生化指标研究
片形吸虫病是一种世界性的寄生虫病,对许多国家的经济和公共卫生造成严重影响。因此,预防、有效治疗和教育运动是预防该病的重要手段。本研究旨在测定阿苯达唑(ABZ)治疗后21天(D0 ~ D21)内肝片吸虫肝损伤的血清生化指标。选取24头自然感染肝片吸虫的奶牛,随机分为对照组(n = 12)和ABZ (7.5 mg / kg体重)处理组(n = 12)。测定两组肝脏酶-天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)和胆汁淤积酶- γ -谷氨酰转移酶(GGT)及碱性磷酸酶(ALP)的活性。两组在实验过程中(D0 ~ D21) AST活性均显著升高(P <0.001)。两组在D0 ~ D21期间ALT活性均显著降低(P <0.001)。对照组GGT活性在实验过程中无明显变化(P >0.05)。相反,治疗组GGT活性显著降低(P <0.01)。治疗组血清GGT活性在D7和D21显著低于对照组(P <0.01)。治疗组血清GGT活性降低可能是由于阿苯达唑消除片形虫和改善胆汁流动的作用。ALP测定结果显示各组间ALP活性无明显变化。由此可以得出结论,肝梭菌引起的胆汁淤积最有用的标志是GGT活性,并有助于评价ABZ对牛的治疗效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信