Effect of Partially Purified Flavonoids from Albizia lebbeck (L) Benth Leaves Against Antibiotic-Resistant Streptococcus Isolates

Abstract

Background:Plant flavonoids have strong biological activity, one of which is their antimicrobial activity. The design of this study, the content of flavonoids was determined in the leaves of the Albizia lebbeck plant, which is considered one of the medicinal plants.Materils and Methods: Extraction of flavonoids from the leaves of Albizia lebbeck ,Qualitative determination of flavonoids using thin-layer chromatography (TLC) of Albizia lebbeck leaf extract and quantitative analysis using high-performance liquid chromatography (HPLC) .Results: showed the appearance of spots of flavonoid groups. Kaempferol, while quantitative analysis using high-performance liquid chromatography (HPLC) showed the presence of Catechin, Gallic acid Epicatechin, Chlorogenic acid as two isomers a & b, Caffaic acid, Luetolin, Apigenin and Querecetin. Partially purified flavonoid extract from Albizia leaves showed a clear inhibition activity against 4 types of Streptococcus bacteria resistant to antibiotics: ampicillin, tetracycline, clarithromycine, clindamycin, and rifampin.Conclusion: These results encourage the possibility of using the active compounds of this plant as safe alternatives against pathogenic isolates of the genus Streptococcus. other bacterial genera.