Detecting QTLs controlling chlorophyll fluorescence parameters in Iranian wheat recombinant inbred lines

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2023-11-10 DOI:10.1016/j.plgene.2023.100437

Narges Sahranavard , Eisa Jorjani , Hossein Sabouri , Sharifeh Mohahamad Alegh , Mahnaz Katouzi

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引用次数: 0

Abstract

Measurement of chlorophyll fluorescence is one of the methods to detect a disorder in the photosynthetic system, which reflects the photochemical state of the plant. Identification of the genetic structure of chlorophyll fluorescence parameters can provide useful solutions for breeding varieties with higher potential. The present study was conducted with the aim of identifying quantitative loci (QTL) related to chlorophyll fluorescence parameters in an Iranian RILs wheat population. One hundred and twenty F8 RILs of wheat derived from crossing Kohdasht (KHD) and Gonbad (GND) cultivars in the form of an alpha lattice design were studied in 2019 and 2020. The analysis of QTLs was performed in the software R using the package QTL.gCIMapping.GUI v2.0. The genetic map was constructed with 423 SSR markers, 21 CBDP markers, 58 ISJ markers, and 19 SCoT markers (521 polymorphic alleles) distributed on 21 wheat chromosomes. This map covered 3167.9 cM of the wheat genome and had an average marker spacing of 6.1, 6, and 6.2 cM for the A, B, and D genomes, respectively. Thirteen of the fourteen QTLs discovered in 2019 and four of the nine QTLs discovered in 2020 had an explanatory coefficient greater than 15% and were considered major QTLs. In 2019, pleiotropic QTLs were found at position 1.79 cM on chromosome 3A and between two markers Xwmc11-3A and BARC1177. In 2020, two QTLs, qABS/ RC 1B and qTRo/ RC 1B were located at position 36.80 cM 1B and between two markers Xgpw4331-1B and Xgpw5162-1B, and two QTLs, qETo/ RC-5D and qREo / RC-5D were located at position 63.25 cM chromosome 5D and correspond to the position of marker cfd266. Pleiotropic QTLs as well as major QTLs can be used in marker-assisted selection for chlorophyll fluorescence traits in wheat breeding projects.

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伊朗小麦重组自交系控制叶绿素荧光参数的qtl检测

叶绿素荧光的测量是检测光合系统紊乱的方法之一，反映了植物的光化学状态。叶绿素荧光参数的遗传结构鉴定可以为选育高潜力品种提供有用的解决方案。本研究的目的是确定与伊朗小麦群体叶绿素荧光参数相关的定量位点(QTL)。2019年和2020年以α晶格设计的形式研究了Kohdasht (KHD)和Gonbad (GND)杂交小麦的120个F8 ril。qtl分析在R软件中使用QTL.gCIMapping.GUI v2.0包进行。共构建了21条小麦染色体上423个SSR标记、21个CBDP标记、58个ISJ标记和19个SCoT标记(521个多态性等位基因)的遗传图谱。该图谱覆盖小麦基因组3167.9 cM, A、B和D基因组的平均标记间距分别为6.1、6和6.2 cM。2019年发现的14个qtl中有13个，2020年发现的9个qtl中有4个解释系数大于15%，被认为是主要qtl。2019年，在3a染色体的1.79 cM位置以及两个标记xwmc11 - 3a和BARC1177之间发现了多角性qtl。在2020年，qABS/ RC 1B和qTRo/ RC 1B两个QTLs位于36.80 cM 1B位置，位于Xgpw4331-1B和Xgpw5162-1B两个标记之间，qETo/ RC-5D和qREo / RC-5D两个QTLs位于5D染色体63.25 cM位置，对应标记cfd266的位置。多效性qtl和主效qtl可用于小麦叶绿素荧光性状的标记辅助选择。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.