Assessment of lymphocytosis among pertussis patients in Northwest Ethiopia

Q3 Medicine

Vacunas Pub Date : 2024-04-01 DOI:10.1016/j.vacun.2023.08.006

Solomon Taye Sima , Debasu Damtie , Belay Tessema , Baye Gelaw , Feleke Moges

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引用次数: 0

Abstract

Background

Pertussis is a severe and prolonged coughing disease caused by Bordetella pertussis. In 2014, 24.1 million pertussis cases and 160 700 deaths were estimated worldwide in children less than 5 years old. Infection with B. pertussis is frequently characterized by a significant rise in the number of circulating lymphocytes. Thus, the objective of this study was to assess lymphocytosis among pertussis patients in Northwest Ethiopia.

Methods

An institution-based cross-sectional study was conducted among pertussis patients who met the clinical case definition for pertussis. The study was conducted from July 2018 through February 2019 and nasopharyngeal swabs were collected from 321 participants, and samples were analyzed using real-time PCR (RT-PCR). Blood specimens were collected from 321 study participants and total lymphocyte count was conducted using hematology analyzer.

Results

A total of 109 participants were positive for Bordetella species. Lymphocytosis was found in 15/109 (13.8%) of the PCR confirmed pertussis patients. Of those 15 PCR confirmed participants with lymphocytosis, 8/15 (53.3%) had lymphocytosis grading ‘high’ (≥ 2.81/μL) followed by 3/15 (20.0%) ‘moderately high’ (≥ 4.0/μL), and 4/15 (26.7%) had ‘very high’ (≥ 6.0/μL). Among PCR confirmed pertussis participants with lymphocytosis, 11/15 (73.3%) were less than 1 year old.

Conclusions

Despite other co-infections can cause lymphocytosis, this study showed that lymphocyte count could be used to rule out pertussis. Thus, in combination with other specific laboratory tests, lymphocyte count in pertussis suspected patients is recommended.

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评估埃塞俄比亚西北部百日咳患者的淋巴细胞增多症

背景百日咳是由百日咳杆菌引起的一种严重而持久的咳嗽疾病。据估计，2014 年全球有 2410 万例百日咳病例和 160 700 例死亡病例发生在 5 岁以下儿童身上。感染百日咳杆菌后，循环淋巴细胞数量通常会显著增加。因此，本研究旨在评估埃塞俄比亚西北部百日咳患者的淋巴细胞增多情况。方法对符合百日咳临床病例定义的百日咳患者进行了一项基于机构的横断面研究。研究于2018年7月至2019年2月进行，收集了321名参与者的鼻咽拭子，并使用实时PCR（RT-PCR）对样本进行分析。从 321 名研究参与者中采集了血液标本，并使用血液分析仪进行了总淋巴细胞计数。在 PCR 确诊的百日咳患者中，有 15/109 人（13.8%）出现淋巴细胞增多。在这15名经PCR确诊的淋巴细胞增多患者中，8/15（53.3%）的淋巴细胞增多分级为 "高"（≥ 2.81/μL），3/15（20.0%）为 "中高"（≥ 4.0/μL），4/15（26.7%）为 "极高"（≥ 6.0/μL）。结论尽管其他合并感染可导致淋巴细胞增多，但本研究表明淋巴细胞计数可用于排除百日咳。因此，建议对百日咳疑似患者进行淋巴细胞计数，并结合其他特异性实验室检查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Vacunas Medicine-Infectious Diseases

CiteScore

3.90

自引率

0.00%

发文量

138

审稿时长

62 days

期刊介绍： Sin duda una de las mejores publicaciones para conocer los avances en el campo de las vacunaciones preventivas, tanto en el ámbito de la investigación básica como aplicada y en la evaluación de programas de vacunaciones. Su alta calidad y utilidad la ha llevado a estar indexada en los prestigiosos índices IME y SCOPUS.