RUMEYSA ÖZYURT, NİLÜFER ERKASAP, METE ÖZKURT, SERDAR MUSTAFA ERKASAP, KONSTANTİNOS DİMAS, AYŞE ÇAKIR GÜNDOĞDU, ENGİN ULUKAYA
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引用次数: 0
Abstract
Background/aim: Colorectal cancer (CRC) is a fatal malignancy type and its occurence still needs to be explored mechanistically. Notch, IL-1, and leptin crosstalk is reported to play a role in the proliferation, migration, and expression of proangiogenic molecules. In this study, we aimed to investigate the effect of inhibition of Notch, IL-1, and leptin on CRC. Materials and methods: To generate colorectal cancer tumor xenografts, 1 × 107 cells from exponentially growing cultures of HCT15 cells were injected subcutaneously, at the axillary region of the left and right rear flanks of forty NOD.CB17-Prkdcscid/J (NOD/SCID) female mice. The mice were then monitored for the development of tumors and were randomly divided into five groups when tumor sizes reached a volume of approximately 150 mm3 . Mice were used to determine the effectiveness of the gamma-secretase inhibitor (DAPT, Notch inhibitor), the interleukin-1 receptor antagonist (Anakinra) and the leptin receptor antagonist (Allo aca) against tumor growth. The mice were euthanized by CO2 inhalation immediately after the treatments finished, and all efforts were made to minimize suffering. Tumors were excissed for RT-PCR and histological analysis. Results: There is an intact Notch, IL-1, and leptin signaling axis, and in vivo antagonism of Notch, IL-1, and leptin affects mRNA and protein expression of inflammatory and angiogenic molecules. Conclusion: Present data suggest that targeting Notch, IL-1, and leptin may be possesses therapeutic potential in CRC.
期刊介绍:
The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological
Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological
processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology,
genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics
and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open
to researchers of all nationalities.