Image-Based Gel Encapsulation of Suspended Single Cells for Parallel Single-Cell Screening

IF 0.9 Q4 ROBOTICS

Journal of Robotics and Mechatronics Pub Date : 2023-10-20 DOI:10.20965/jrm.2023.p1177

Venkatesh Kumar Panneer Selvam, Muhammad Luqman Arief Bin Kamaludin, Ghulam Murtaza, Rifat Hussain Chowdhury, Tanmay Debnath, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

引用次数: 0

Abstract

Single-cell screening, which has revolutionized the life sciences, is an important method for detecting, separating, or treating specific cells based on desired characteristics. Previously, single cells of interest were manually identified in an image, which required human labor and time. We developed an automated photopolymerization system to encapsulate suspended single cells in approximately 50-µm photo-crosslinkable hydrogel squares. An image was captured, and single cells were selected from grouped cells based on image processing. A generated image was transferred to a digital micromirror device (DMD), and in parallel, target-suspended single cells were encapsulated in gelatin methacryloyl (GelMA) hydrogels. We built a data transfer platform based on a Power Automate Desktop (PAD), completed the data transfer, and projected the processed image onto a sample in 10 s, ensuring a minimum alignment error of 6.2 µm.

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基于图像的悬浮单细胞凝胶包封技术用于单细胞平行筛选

单细胞筛选，已经彻底改变了生命科学，是一种重要的方法来检测，分离，或处理特定的细胞基于所需的特征。以前，单个感兴趣的细胞是在图像中手动识别的，这需要人力和时间。我们开发了一种自动化光聚合系统，将悬浮的单细胞封装在大约50µm的光交联水凝胶正方形中。捕获图像，并根据图像处理从分组细胞中选择单个细胞。生成的图像被传输到数字微镜装置(DMD)，同时，目标悬浮的单细胞被包裹在明胶甲基丙烯酰(GelMA)水凝胶中。搭建了基于Power automation Desktop (PAD)的数据传输平台，在10 s内完成数据传输，并将处理后的图像投影到样品上，保证了最小对准误差为6.2µm。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Robotics and Mechatronics ROBOTICS-

CiteScore

2.20

自引率

36.40%

发文量

134

期刊介绍： First published in 1989, the Journal of Robotics and Mechatronics (JRM) has the longest publication history in the world in this field, publishing a total of over 2,000 works exclusively on robotics and mechatronics from the first number. The Journal publishes academic papers, development reports, reviews, letters, notes, and discussions. The JRM is a peer-reviewed journal in fields such as robotics, mechatronics, automation, and system integration. Its editorial board includes wellestablished researchers and engineers in the field from the world over. The scope of the journal includes any and all topics on robotics and mechatronics. As a key technology in robotics and mechatronics, it includes actuator design, motion control, sensor design, sensor fusion, sensor networks, robot vision, audition, mechanism design, robot kinematics and dynamics, mobile robot, path planning, navigation, SLAM, robot hand, manipulator, nano/micro robot, humanoid, service and home robots, universal design, middleware, human-robot interaction, human interface, networked robotics, telerobotics, ubiquitous robot, learning, and intelligence. The scope also includes applications of robotics and automation, and system integrations in the fields of manufacturing, construction, underwater, space, agriculture, sustainability, energy conservation, ecology, rescue, hazardous environments, safety and security, dependability, medical, and welfare.