Screening and Characterization of Multidrug Resistant Bacteria from Chronic Kidney Disease Patients of Warangal

Abstract

With an overall incidence of over 10% within regular population, Chronic kidney disease is an issue that is becoming more and more important in terms of public health. The enhanced risk of infection, especially those brought on by bacteria that are multi-drug resistant, is one of the main side effects of chronic kidney disease. It is essential to screen and identify multidrug resistant bacteria in chronic kidney disease patients, especially those receiving haemodialysis, in order to prevent the transmission of these pathogens. Hence, to improve outcomes for chronic kidney disease patients, early diagnosis and prompt treatment of drug-resistant bacteria are essential. A total of 2219 samples were screened for antibiotic resistant microbes in hospital samples. 445 samples tested positive (20.05 %) for bacterial growth and 1774 samples tested negative (79.94 %). The rate of multidrug resistance bacterial infections was 17% and 43% higher in CKD patients for estimated glomerular filtration rate between 30 and 59 ml/min/1.73m2 and glomerular filtration rate 30 ml/min/1.73m2 respectively. Five bacterial isolates were found to exhibit multi-antibiotic resistance. The Multiple Antibiotic Resistance (MAR) Index ranged from 0.3 to 0.7 across the isolates. The isolates were identified as Enterobacter bugandensis, Enterococcus faecium, Providencia stuartii, Klebsiella variicola, and Escherichia coli by 16S rRNA gene sequencing and phylogenetic analysis. In conclusion, screening and identification of multidrug resistance bacteria is essential to prevent and control the spread of these pathogens and will be helpful for the effective treatment of the multidrug resistance in chronic kidney disease patients.