Bioanalytical Studies on Fungal Pathogens Originating from Spoiled Alpan Banana (Musa Paradisiaca)

Amrendra Kumar singh, Prakash Chandra Gupta
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Abstract

Background: The entire Alpan banana plant can be utilized medicinally. Since the banana's rapid global expansion, several non-wood plant fibers have been used as substitutes for wood pulp in the manufacture of pulp, paper, and paper board. Currently, modest commercial pulping operations use a range of non-wood fibers, including bamboo, jute, straw, rice, and abaca. The increasing number of banana varieties is a result of the everyday developments that are being achieved in several fields. The Materials and Methods: The purpose of this current study is to check fungal pathogenicity in infected Alpan banana fruit, molecular characterization of the pathogenic species and evaluation of biochemical activities in banana fruit. The studied biochemical activities include protein content, ascorbic acid, total sugar and total phenolic compounds. Ascorbic acid oxidase (AAO) and Phenol Oxidase (PPO) are also studied in the selected banana variety. Result: The fungal pathogenicity assay showed incidence of Aspergillus species on Alpan banana fruit. A very high prevalence of Aspergillus flavus and Aspergillus fumigatus were observed in examined fruits. Species confirmation were based on molecular characterization method and its subsequent bioinformatic analysis. Proteins, total phenolic content and Keto acid were observed to be 223.01, 47.32 and 964.0 mg/g respectively whereas PPO and AAO showed 0.800 and 0.175 U/ml content in the fruit sample. Conclusion:The study concludes the prevalence of fungal infection in ripe bananas by Aspergillus species and changes observed at their biochemical constituents level post fungal infestation
变质阿尔卑斯香蕉(Musa Paradisiaca)病原菌的生物分析研究
背景:整个阿尔卑斯香蕉植物都可以药用。自从香蕉在全球迅速扩张以来,一些非木质植物纤维已被用作木浆的替代品,用于制造纸浆、纸张和纸板。目前,适度的商业制浆业务使用一系列非木材纤维,包括竹、黄麻、稻草、大米和abaca。香蕉品种数量的增加是几个领域每天都在取得发展的结果。材料与方法:本研究旨在检测真菌对阿尔卑斯香蕉果实的致病性、病原菌的分子特征及对香蕉果实生化活性的评价。研究的生化活性包括蛋白质含量、抗坏血酸、总糖和总酚类化合物。还对所选香蕉品种的抗坏血酸氧化酶(AAO)和酚氧化酶(PPO)进行了研究。结果:真菌致病性测定显示,阿尔卑斯香蕉果实中存在曲霉种。黄曲霉和烟曲霉在水果中的流行率很高。物种确认是基于分子鉴定方法和随后的生物信息学分析。蛋白质、总酚和酮酸含量分别为223.01、47.32和964.0 mg/g, PPO和AAO含量分别为0.800和0.175 U/ml。结论:研究了成熟香蕉中曲霉侵染的流行程度,以及侵染后香蕉生化成分水平的变化
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