Discrepancy of Beta-Hydroxybutyrate Measurements between a Blood Meter and GC-MS Methods in Healthy Humans

IF 0.5 Q4 ORTHOPEDICS
Angelia Maleah Holland-Winkler, Andrew R. Moore, Jenna K. Ansley, Noah A. Fritz, Ilya Bederman
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引用次数: 0

Abstract

Ketone salt (KS) supplementation induces temporary nutritional ketosis to achieve potential exercise performance and health benefits. Racemic KS includes both D/L isomers of β-hydroxybutyrate, yet commercially available measurement devices (i.e., blood meters) only measure the D variant. The aim of this study was to investigate the efficacy of a blood meter to measure serum β-hydroxybutyrate in comparison with gas chromatography–mass spectrometry (GC-MS) before and 30 min after consuming a placebo or racemic KS. In this triple-blinded cross-over study, 16 healthy adults were administered either a placebo or KS drink, and the circulating β-hydroxybutyrate concentration was measured at baseline (PRE) and 30 min following consumption (POST) using a blood ketone meter and by GC-MS. Compared to the placebo, both GC-MS and the blood meter obtained significantly greater β-hydroxybutyrate levels from PRE to POST time-points after consuming KS. Additionally, GC-MS results showed significantly higher levels of β-hydroxybutyrate with both the placebo and KS at PRE and POST time-points, as compared to the blood meter. These results indicate that (1) even in the absence of KS, the blood meter yields significantly lower β-hydroxybutyrate values than GC-MS, and (2) the inability of the blood meter to measure L-β-hydroxybutyrate values POST KS warrants the further development of publicly available ketone measurement apparatuses.
健康人血液计与气相色谱-质谱法测定β -羟基丁酸的差异
酮盐(KS)补充诱导暂时的营养性酮症,以实现潜在的运动表现和健康益处。外消旋KS包括β-羟基丁酸酯的D/L异构体,但商业上可用的测量设备(即血液计)只能测量D变体。本研究的目的是研究血仪在服用安慰剂或消旋KS前和服用后30分钟测量血清β-羟基丁酸的效果,并与气相色谱-质谱法(GC-MS)进行比较。在这项三盲交叉研究中,16名健康成年人被给予安慰剂或KS饮料,并在基线(PRE)和消费后30分钟(POST)使用血酮仪和气相色谱-质谱法测量循环β-羟基丁酸浓度。与安慰剂相比,服用KS后,从PRE到POST时间点,GC-MS和血压计的β-羟基丁酸水平均显著提高。此外,GC-MS结果显示,与血液计相比,安慰剂组和KS组在术前和术后时间点的β-羟基丁酸水平明显更高。这些结果表明:(1)即使在没有KS的情况下,血液计的β-羟基丁酸值也明显低于GC-MS,(2)血液计无法测量L-β-羟基丁酸值,因此需要进一步开发公开可用的酮类测量仪器。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
51
期刊介绍: MLTJ (Muscle, Ligaments and Tendons Journal) is an open access, peer-reviewed online journal that encompasses all aspects of clinical and basic research studies related to musculoskeletal, ligament, tendon, public health, exercise physiology and kinesiology issues. Muscle, Ligaments and Tendons Journal (MLTJ) provides the platform for exchange of new clinical and scientific information in the most precise and expeditious way to achieve timely dissemination of information and cross-fertilization of ideas. It is the official journal of the Italian Society of Muscles, Ligaments and Tendons (I.S.Mu.L.T.), Società Italiana Terapia con Onde D’urto (S.I.T.O.D.) and Società Italiana Studio Piede e Caviglia (S.I.S.P.E.C)
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