Formulation, development and evaluation of Woodfordia fruticosa and β-sitosterol mediated silver nanoparticles gel for synergistic effect

Abstract

Background

Silver nanoparticles (AgNP) are frequently utilized metallic nanoparticles in healthcare systems. In this investigation, AgNP was produced employing β-sitosterol and Woodfordia fruticosa gel for the purpose of ameliorating wound healing.

Methods

The characterization of AgNP was conducted via SEM and spectrophotometry analysis. Simultaneously, a solution with a concentration of 1 ​mM of silver nitrate was prepared by dissolving 16.99 ​mg of silver nitrate in 100 ​ml of distilled water. Subsequently, the API and the silver nitrate solution were utilized in the synthesis of silver nanoparticles. The physicochemical properties, stability, and morphology of the synthesized silver nanoparticles were evaluated through various techniques, such as zeta potential analysis and electron microscopy. These techniques were employed for the purpose of assessing the aforementioned properties. Following the gel preparation, an assessment of the gel was performed.

Result

The investigation successfully produced silver nanoparticles (AgNP) using β-sitosterol and Woodfordia fruticosa gel. To characterize the synthesized AgNP, various techniques were employed, including SEM and spectrophotometry analysis. A 1 ​mM solution of silver nitrate was prepared and used in the synthesis process, and the physicochemical properties, stability, and morphology of the AgNP were assessed through zeta potential analysis and electron microscopy.

Conclusion

The results of this study indicate the successful synthesis of silver nanoparticles using β-sitosterol and Woodfordia fruticosa gel. The characterization techniques, including SEM and spectrophotometry, confirmed the formation of AgNP. The analysis of physicochemical properties and stability, particularly through zeta potential analysis, provided valuable insights into the stability of the synthesized nanoparticles. These findings have significant implications in the context of wound healing. Silver nanoparticles have been widely studied for their potential wound healing properties due to their antimicrobial and anti-inflammatory characteristics. The utilization of natural compounds like β-sitosterol and botanical sources like Woodfordia fruticosa gel for the synthesis of AgNP is particularly promising as it may reduce potential toxicity and enhance the biocompatibility of these nanoparticles.