Morphology and metabolism of storage substances contribution to alkali stress responses in two contrasting barley cultivars during germination stage

IF 1.1 4区 农林科学 Q3 AGRONOMY
Jianbo Li, Yanan Jin, Zhiping Liu, Tu Ba, Wendi Wang, Shoujun Xu
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引用次数: 0

Abstract

Abstract: Barley (Hordeum vulgare L.) is a globally significant crop and serves as a pioneer crop for improving saline-alkaline soils due to its salt-alkali tolerant properties. However, the response mechanism of barley to alkali stress remains unclear. In this study, two barley genotypes with contrasting performance under alkali stress were selected: the alkali-tolerant SCMS and the alkali-sensitive QT9919. The morphological and physiological mechanisms of these two barley cultivars in response to alkali stress were elucidated. Results showed that alkali stress inhibited germination in QT9919, leading to significant decreases in germination vigor, plant height and root length. On the other hand, SCMS presented stronger water absorbing capacity than QT9919 under all circumstances. Furthermore, starch degradation and sugar metabolism were suppressed by alkali stress in both barley cultivars through down-regulation of genes expression and reduction of enzymes activities involved in the processes of EMP (Embden-Meyerhof-Parnas), tricarboxylic acid cycle (TCA), and pentose phosphate pathway (PPP). Notably, enzyme activity of hexokinase (HK) and malic dehydrogenase (MDH) showed significant changes under alkali stress between the two barley genotypes. This study provides insights into screening barley germplasm with strong alkali-tolerance and holds significant implications for improving and utilizing of saline-alkaline soils.
两个对比品种萌发期贮藏物质形态和代谢对碱胁迫响应的影响
摘要:大麦(Hordeum vulgare L.)因其耐盐碱特性而成为改良盐碱地的先锋作物,是全球重要作物。然而,大麦对碱胁迫的响应机制尚不清楚。本研究选择了耐碱的SCMS和碱敏感的QT9919两个在碱胁迫下表现不同的大麦基因型。阐明了这两个大麦品种对碱胁迫的形态和生理机制。结果表明,碱胁迫抑制了QT9919的萌发,导致萌发活力、株高和根长显著降低。另一方面,在所有条件下,SCMS均表现出比QT9919更强的吸水能力。此外,碱胁迫通过下调EMP (Embden-Meyerhof-Parnas)、三羧酸循环(TCA)和戊糖磷酸途径(PPP)相关基因表达和酶活性,抑制了两种大麦淀粉降解和糖代谢。在碱胁迫下,两种大麦基因型的己糖激酶(HK)和苹果脱氢酶(MDH)活性均发生了显著变化。本研究为大麦耐碱性强种质的筛选提供了新的思路,对盐碱地改良和利用具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Seed Science
Journal of Seed Science Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
2.00
自引率
30.00%
发文量
28
审稿时长
12 weeks
期刊介绍: From 2017 the Journal of Seed Science (JSS) will circulate online version only. Original scientific studies and communications, not yet published or submitted to another journal for publication and written in Portuguese or English, will be accepted for publication. For manuscripts submitted in English, the authors should provide an adequated version. The SCIENTIFIC COMMUNICATION is a category of scientific manuscript which describes a technique, an equipment, new species or observations and surveys of limited results. It has the same scientific rigor as the “Scientific Articles” and the same value as a publication. The classification of a manuscript as a SCIENTIFIC COMMUNICATION is based on its content and scientific merit but it can be a preliminary study, simple and not definitive on a certain subject, with publication justified by its uniqueness and contribution to the area. The Editorial Board of the JSS may invite leading authors of recognized reputation to compose specific Review Articles covering topics of their specialization that will convey to the scientific community the state-of-the-art knowledge related to the specific theme.
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