Rice bran stabilization using autoclave and optimization of crude rice bran oil recovery using ultrasound-assisted extraction

IF 1.3 4区农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD

Bioresources Pub Date : 2023-10-22 DOI:10.15376/biores.18.4.8341-8361

Dodyk Pranowo, Tsamara Dhany Savira, None Sukardi

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Abstract

This research aimed to stabilize rice bran to reduce enzymatic reactions and optimize rice bran oil extraction using ultrasound-assisted extraction (UAE). The optimization was performed using response surface methodology (RSM). The results showed that rice bran oil has three primary fatty acids (oleic, linoleic, palmitic). Stabilization of rice bran using an autoclave (121 °C) for 10 min was recommended, with oil yield (14.2%), free fatty acid/FFA (0.41%), and gamma oryzanol (16.1 ppm). The storage significantly affected oil yield and gamma oryzanol but not FFA (p>0.05). The untreated and stabilized rice bran FFA during 18-day storage ranged from 1.33 to 5.73% and 0.41 to 1.12%. This confirmed that stabilization could inactivate lipase enzymes by reducing FFA. The optimization showed that the linear model best explained oil yield, total phenol content (TPC), and antioxidant activity (IC50), while the 2FI model was best fitted for gamma oryzanol. The optimal condition was found for UAE extraction at an amplitude of 90% and a time of 30 min. Verifying optimal conditions resulted in oil yield (13.6%), TPC (44.8 mg GAE/g), IC50 (207.2 ppm), and gamma oryzanol (15.8 ppm). Further in-depth studies are required to investigate using green solvents in the UAE and gamma oryzanol purification.

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蒸压釜稳定米糠及超声辅助提取粗米糠油的优化

本研究旨在稳定米糠，减少酶促反应，优化超声辅助提取米糠油的工艺。采用响应面法(RSM)进行优化。结果表明，米糠油含有油酸、亚油酸和棕榈酸三种主要脂肪酸。推荐使用高压灭菌器(121°C)稳定米糠10分钟，出油率为14.2%，游离脂肪酸/FFA为0.41%，γ -米甲醇为16.1 ppm。贮藏对油脂产量和γ -米甲醇有显著影响，对游离脂肪酸无显著影响(p>0.05)。贮藏18 d时，未处理和稳定的米糠游离脂肪酸含量分别为1.33 ~ 5.73%和0.41 ~ 1.12%。这证实了稳定化可以通过减少FFA来灭活脂肪酶。优化结果表明，线性模型最能解释出油率、总酚含量(TPC)和抗氧化活性(IC50)，而2FI模型最能解释γ -米扎酚。最佳提取条件为提取率为90%，提取时间为30 min。对最佳提取条件进行验证，得到出油率(13.6%)，TPC (44.8 mg GAE/g)， IC50 (207.2 ppm)和γ -米zanol (15.8 ppm)。需要进一步深入的研究，以探讨在阿联酋使用绿色溶剂和γ -米甲醇净化。

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来源期刊

Bioresources 工程技术-材料科学：纸与木材

CiteScore

2.90

自引率

13.30%

发文量

397

审稿时长

2.3 months

期刊介绍： The purpose of BioResources is to promote scientific discourse and to foster scientific developments related to sustainable manufacture involving lignocellulosic or woody biomass resources, including wood and agricultural residues. BioResources will focus on advances in science and technology. Emphasis will be placed on bioproducts, bioenergy, papermaking technology, wood products, new manufacturing materials, composite structures, and chemicals derived from lignocellulosic biomass.