Auraptene alleviates inflammatory injury and cell apoptosis in children with pneumonia in vitro

IF 2.5 4区医学 Q3 ALLERGY

Allergologia et immunopathologia Pub Date : 2023-11-01 DOI:10.15586/v51i6.974

Yuebin Wang, Shuzhen Yuan, Wei Tan, Yuanyu Zhou, Ruiyun Liao, Wei Su

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引用次数: 0

Abstract

Objective: The aim of the present study is to investigate the effects of auraptene on inflammation and apoptosis of pneumonia cell model and uncover the mechanism. Methods: WI-38 cells were treated with lipopolysaccharide (LPS) to construct a pneumonia model. Cell counting kit-8 assay, enzyme-linked-immunosorbent serologic assay, and quantitative polymerase chain reaction assay were conducted to confirm the effects of auraptene on the viability and inflammation of WI-38 cells. Flow cytometry (FCM) and immunoblot assays were conducted to detect the effects of auraptene on the apoptosis of WI-38 cells. Immunoblot assay was performed to confirm the mechanism. Results: We found that auraptene stimulated cell viability in WI-38 cells upon LPS treatment. Auraptene also inhibited cellular inflammation. Furthermore, auraptene inhibited cell apoptosis of WI-38 cells upon LPS treatment. Mechanically, auraptene inhibited the nuclear factor kappa B signaling pathway, thereby suppressing the pneumonia. Conclusion: Auraptene alleviates inflammatory injury and cell apoptosis in pneumonia, thus has the potential to act as a pneumonia drug.

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auraptenin在体外减轻肺炎患儿炎症损伤和细胞凋亡

目的:探讨auraptene对肺炎细胞模型炎症和凋亡的影响，并揭示其作用机制。方法:采用脂多糖(LPS)处理WI-38细胞，建立肺炎模型。通过细胞计数试剂盒-8、酶联免疫吸附血清学、定量聚合酶链反应验证auraptene对WI-38细胞活力和炎症的影响。采用流式细胞术(FCM)和免疫印迹法检测auraptene对WI-38细胞凋亡的影响。免疫印迹法证实其作用机制。结果:我们发现auraptene对LPS处理后的WI-38细胞有刺激作用。Auraptene还能抑制细胞炎症。此外，auraptene对LPS处理后的WI-38细胞凋亡有抑制作用。机械上，auraptene抑制核因子κ B信号通路，从而抑制肺炎。结论:Auraptene可减轻肺炎的炎症损伤和细胞凋亡，具有作为抗肺炎药物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Allergologia et immunopathologia 医学-过敏

CiteScore

3.70

自引率

0.00%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.