Lauric acid reduces apoptosis by inhibiting FOXO3a-signaling in Deoxynivalenol-treated IPEC-J2 cells

IF 2.7 3区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Animal Science and Technology Pub Date : 2023-09-04 DOI:10.5187/jast.2023.e92

Na Yeon Kim, Sang In Lee

{"title":"Lauric acid reduces apoptosis by inhibiting FOXO3a-signaling in Deoxynivalenol-treated IPEC-J2 cells","authors":"Na Yeon Kim, Sang In Lee","doi":"10.5187/jast.2023.e92","DOIUrl":null,"url":null,"abstract":"Deoxynivalenol (DON) is the most common mycotoxin contaminant of food or feed worldwide and causes disease in animals. Lauric acid (LA) is a medium-chain fatty acid useful for barrier functions such as antimicrobial activity in the intestine of monogastric animals. However, the molecular mechanisms by which lauric acid exerts its effects on the deoxynivalenol-exposed small intestine have not been studied. We used an intestinal porcine epithelial cell line (IPEC-J2) as an <italic>in vitro</italic> model to explore the molecular mechanism of lauric acid in alleviating deoxynivalenol-induced damage. We found that lauric acid reversed deoxynivalenol-induced reduction in cell viability. Our qRT-PCR results indicated that lauric acid alleviated deoxynivalenol-induced apoptosis through Annexin-V. Additionally, immunofluorescence and Western blotting showed that lauric acid attenuated deoxynivalenol-induced Forkhead box O3 (FOXO3a) translocation. These results suggest that lauric acid attenuates Forkhead box O3 translocation in the small intestine damaged by deoxynivalenol, thereby reducing apoptosis. In conclusion, this study found that lauric acid alleviates deoxynivalenol-induced damage in intestinal porcine epithelial cell line through various molecular mechanisms.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"26 1","pages":"0"},"PeriodicalIF":2.7000,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Animal Science and Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5187/jast.2023.e92","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Deoxynivalenol (DON) is the most common mycotoxin contaminant of food or feed worldwide and causes disease in animals. Lauric acid (LA) is a medium-chain fatty acid useful for barrier functions such as antimicrobial activity in the intestine of monogastric animals. However, the molecular mechanisms by which lauric acid exerts its effects on the deoxynivalenol-exposed small intestine have not been studied. We used an intestinal porcine epithelial cell line (IPEC-J2) as an in vitro model to explore the molecular mechanism of lauric acid in alleviating deoxynivalenol-induced damage. We found that lauric acid reversed deoxynivalenol-induced reduction in cell viability. Our qRT-PCR results indicated that lauric acid alleviated deoxynivalenol-induced apoptosis through Annexin-V. Additionally, immunofluorescence and Western blotting showed that lauric acid attenuated deoxynivalenol-induced Forkhead box O3 (FOXO3a) translocation. These results suggest that lauric acid attenuates Forkhead box O3 translocation in the small intestine damaged by deoxynivalenol, thereby reducing apoptosis. In conclusion, this study found that lauric acid alleviates deoxynivalenol-induced damage in intestinal porcine epithelial cell line through various molecular mechanisms.

查看原文本刊更多论文

月桂酸通过抑制脱氧雪腐酚处理的IPEC-J2细胞的foxo3a信号通路减少细胞凋亡

脱氧雪腐镰刀菌醇(DON)是世界范围内食品或饲料中最常见的霉菌毒素污染物，可引起动物疾病。月桂酸(LA)是一种中链脂肪酸，在单胃动物的肠道中具有屏障功能，如抗菌活性。然而，月桂酸对暴露于脱氧雪腐镰刀醇的小肠产生影响的分子机制尚未得到研究。我们使用猪肠上皮细胞系(IPEC-J2)作为体外研究对象。建立模型，探讨月桂酸减轻脱氧雪腐酚损伤的分子机制。我们发现月桂酸逆转脱氧雪梨酚诱导的细胞活力降低。我们的qRT-PCR结果显示月桂酸通过Annexin-V减轻脱氧雪腐酚诱导的细胞凋亡。此外，免疫荧光和免疫印迹显示月桂酸能减弱脱氧雪腐酚诱导的叉头盒O3 (FOXO3a)易位。上述结果提示月桂酸可减弱脱氧雪腐酚损伤小肠的叉头盒O3易位，从而减少细胞凋亡。综上所述，本研究发现月桂酸可通过多种分子机制减轻脱氧雪腐镰刀醇对猪肠上皮细胞系的损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Animal Science and Technology Agricultural and Biological Sciences-Food Science

CiteScore

4.50

自引率

8.70%

发文量

审稿时长

7 weeks

期刊介绍： Journal of Animal Science and Technology (J. Anim. Sci. Technol. or JAST) is a peer-reviewed, open access journal publishing original research, review articles and notes in all fields of animal science. Topics covered by the journal include: genetics and breeding, physiology, nutrition of monogastric animals, nutrition of ruminants, animal products (milk, meat, eggs and their by-products) and their processing, grasslands and roughages, livestock environment, animal biotechnology, animal behavior and welfare. Articles generally report research involving beef cattle, dairy cattle, pigs, companion animals, goats, horses, and sheep. However, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will also be considered for publication. The Journal of Animal Science and Technology (J. Anim. Technol. or JAST) has been the official journal of The Korean Society of Animal Science and Technology (KSAST) since 2000, formerly known as The Korean Journal of Animal Sciences (launched in 1956).