Assessment of Photosensitizer Concentration Effects on the Efficacy of Photodynamic Therapy

IF 1.4 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Journal of lasers in medical sciences Pub Date : 2023-11-08 DOI:10.34172/jlms.2023.53

Reza Vafaee, Babak Arjmand, Maryam Hamzeloo-Moghadam, Mostafa Rezaei Tavirani, Zahra Razzaghi, Reza M Robati, Mitra Rezaei, Fatemeh Montazer

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引用次数: 0

Abstract

Introduction: Photodynamic therapy (PDT) is an attractive approach in medicine. Due to its noninvasive nature and low side effects, PDT has been developed quickly. In the present study, the gene expression profiles of the human cell line that was treated via PDT in the sub-lethal concentration (LC50) and super-lethal concentration (LC90) of a photosensitizer (PS) from Gene Expression Omnibus (GEO) were extracted and the common differentially expressed genes (DEGs) were investigated. Methods: The gene expression profiles of the treated cells were compared with a control, and the common DEGs were determined. The common DEGs were assessed via protein-protein interaction (PPI) network analysis, and gene ontology enrichment was evaluated. The related biological terms for the common genes were identified. Results: Ninety-four common DEGs were selected to be analyzed. It appeared that the activation and increment of gene expression were prominent processes. Jun, Dusp1, Atf4, and Atf3 as four critical genes were highlighted. "Chromosomal and microsatellite instability in colorectal cancer" was identified as the main class of biological terms related to the assessed DEGs. Conclusion: The major molecular events which happened in both analyses indicated that PDT, independent from the concentration of PS, induced gross molecular changes such as the upregulation of Jun and Dusp1.

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光敏剂浓度对光动力治疗效果影响的评价

光动力疗法(PDT)是一种很有吸引力的医学方法。由于其无创性和低副作用，PDT得到了迅速的发展。本研究提取了来自gene expression Omnibus (GEO)的一种光敏剂(PS)在亚致死浓度(LC50)和超致死浓度(LC90)下经PDT处理的人细胞系的基因表达谱，并研究了常见差异表达基因(DEGs)。方法:将处理细胞的基因表达谱与对照组进行比较，并测定常见的deg。通过蛋白质-蛋白质相互作用(PPI)网络分析对常见的deg进行评估，并对基因本体富集进行评估。对常见基因的相关生物学术语进行了鉴定。结果:选取94例常见deg进行分析。基因表达的激活和增加是一个突出的过程。Jun、Dusp1、Atf4和Atf3是四个关键基因。“结直肠癌的染色体和微卫星不稳定性”被确定为与评估的deg相关的主要生物学术语。结论:两个分析中发生的主要分子事件表明，PDT诱导Jun和Dusp1上调等总体分子变化不依赖于PS的浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of lasers in medical sciences RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-

CiteScore

3.00

自引率

13.30%

发文量

期刊介绍： The "Journal of Lasers in Medical Sciences " is a scientific quarterly publication of the Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences. This journal received a scientific and research rank from the national medical publication committee. This Journal accepts original papers, review articles, case reports, brief reports, case series, photo assays, letters to the editor, and commentaries in the field of laser, or light in any fields of medicine such as the following medical specialties: -Dermatology -General and Vascular Surgery -Oncology -Cardiology -Dentistry -Urology -Rehabilitation -Ophthalmology -Otorhinolaryngology -Gynecology & Obstetrics -Internal Medicine -Orthopedics -Neurosurgery -Radiology -Pain Medicine (Algology) -Basic Sciences (Stem cell, Cellular and Molecular application and physic)