{"title":"Engineering of Layered Nanocarriers of Quercetin for the Treatment of Diabetes Using Box‐Behnken Design","authors":"Priyank Shah, Pravin Shende","doi":"10.1002/ppsc.202300037","DOIUrl":null,"url":null,"abstract":"Abstract Layered nanocarriers are polymeric systems integrated with layers of glucose‐responsive polymers and polyethylene glycol monomethyl ether 2000 PEG 2000 to enhance the targeting effect and release kinetics. The prime purpose of this research study is to demonstrate a controlled release of Quercetin from formulation of quercetin (Qu)‐based layered nanocarriers (LNCs) with higher stability and anti‐diabetic activity. The QuLNCs are synthesized using the nanoprecipitation method and optimized by Box‐Behnken design of the Design of Experiment (DoE) method. The Quercetin Nanoparticles (QuNPs) are prepared using Polyvinyl alcohol (PVA) and Poly (D,L‐ lactic‐co‐glycolic acid) (PLGA) polymers where the two layers of Phenylboronic acid (PBA) are conjugated using 3‐amino propyl‐triethoxysilane (APTES) as a functionalizing agent followed by PEGylation of the entire system using PEG 2000 . The optimized QuLNCs are characterized by various parameters like Particle size (PS), Zeta potential (ZP), % Entrapment efficiency (%EE), Attenuated total reflectance‐ Fourier Transform Infrared Spectroscopy (ATR‐FTIR), Differential scanning Calorimetry (DSC), Transmission electron microscopy (TEM), in vitro as well as in vivo studies. The QuLNCs showed a % entrapment efficiency of 82.846 ± 0.957% and release of 85.04 ± 3.21% of Qu for 24 h from the layered nanocarriers. The in vivo studies of QuLNCs exhibited a significant controlled release of quercetin for modulating blood glucose levels. Hence, these results proved QuLNCs system acts as a favorable approach for the treatment of type 1 diabetes to offer a longer duration of action.","PeriodicalId":19903,"journal":{"name":"Particle & Particle Systems Characterization","volume":"13 3","pages":"0"},"PeriodicalIF":2.7000,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Particle & Particle Systems Characterization","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/ppsc.202300037","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract Layered nanocarriers are polymeric systems integrated with layers of glucose‐responsive polymers and polyethylene glycol monomethyl ether 2000 PEG 2000 to enhance the targeting effect and release kinetics. The prime purpose of this research study is to demonstrate a controlled release of Quercetin from formulation of quercetin (Qu)‐based layered nanocarriers (LNCs) with higher stability and anti‐diabetic activity. The QuLNCs are synthesized using the nanoprecipitation method and optimized by Box‐Behnken design of the Design of Experiment (DoE) method. The Quercetin Nanoparticles (QuNPs) are prepared using Polyvinyl alcohol (PVA) and Poly (D,L‐ lactic‐co‐glycolic acid) (PLGA) polymers where the two layers of Phenylboronic acid (PBA) are conjugated using 3‐amino propyl‐triethoxysilane (APTES) as a functionalizing agent followed by PEGylation of the entire system using PEG 2000 . The optimized QuLNCs are characterized by various parameters like Particle size (PS), Zeta potential (ZP), % Entrapment efficiency (%EE), Attenuated total reflectance‐ Fourier Transform Infrared Spectroscopy (ATR‐FTIR), Differential scanning Calorimetry (DSC), Transmission electron microscopy (TEM), in vitro as well as in vivo studies. The QuLNCs showed a % entrapment efficiency of 82.846 ± 0.957% and release of 85.04 ± 3.21% of Qu for 24 h from the layered nanocarriers. The in vivo studies of QuLNCs exhibited a significant controlled release of quercetin for modulating blood glucose levels. Hence, these results proved QuLNCs system acts as a favorable approach for the treatment of type 1 diabetes to offer a longer duration of action.
期刊介绍:
Particle & Particle Systems Characterization is an international, peer-reviewed, interdisciplinary journal focusing on all aspects of particle research. The journal joined the Advanced Materials family of journals in 2013. Particle has an impact factor of 4.194 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)).
Topics covered include the synthesis, characterization, and application of particles in a variety of systems and devices.
Particle covers nanotubes, fullerenes, micelles and alloy clusters, organic and inorganic materials, polymers, quantum dots, 2D materials, proteins, and other molecular biological systems.
Particle Systems include those in biomedicine, catalysis, energy-storage materials, environmental science, micro/nano-electromechanical systems, micro/nano-fluidics, molecular electronics, photonics, sensing, and others.
Characterization methods include microscopy, spectroscopy, electrochemical, diffraction, magnetic, and scattering techniques.