Investigation Extraction of the Garden Snail's Shells using GC-MS Spectrometry

Abdulrazzaq B Kadhim, Hazem Almhanna
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Abstract

This study focused on the powder of the shell of the local garden snail in Iraq to identify the chemical compounds and determine the diversity and biological contents that might be applied for other applications and studies. Thus, snails shell was collected from local rivers in Al Diwaniya city, middle of Iraq; 500 grams of the shell powder of the garden snail was smashed and grinded and analysed during this study. The shell powder was extracted by ethanol, and 250mg of the yield was injected in GC-Mass spectrometry and analyzed. The detection of chemical compounds in the garden snail shell was identified by comparison with the mass spectra library of the GC-Mass data system. Results exhibited that six chemical compounds were largely predominant among other elements of the shell powder, including hexadecanoic acid, ethyl ester, (E)-9-Octadecenoic acid ethyl ester, pentadecanoic acid, heptadecanoic acid, ethyl ester, and 9-Octadecenamide, (Z)-. Also, the analysis profiled more than 23 chemical compounds that constituted the structure of the garden snail shell with a low percentage area. These substances were classified into fatty acids, saturated fatty acids, organic compounds, natural products, and methyl and ethyl esters. Consequently, these natural materials are essential active compounds that could be used for more medical and research applications studies. However, the yield of chemical compounds was a low amount. To sum up, GC-Mass spectrometry has displayed a high biological diversity of chemical compounds substances in the shell of garden snails, which can help researchers to investigate more for different natural chemical resources in the shell of a garden snail.
气相色谱-质谱联用法提取田螺壳的研究
本研究对伊拉克当地园林蜗牛壳粉末进行了化学成分鉴定,确定了其多样性和生物学含量,为其他应用和研究提供了可能。因此,从伊拉克中部Al Diwaniya市的当地河流中收集蜗牛壳;在本研究中,对500克园螺壳粉进行了粉碎、研磨和分析。用乙醇提取壳粉,取产率250mg进行gc -质谱分析。通过与GC-Mass数据系统的质谱库比对,确定了花园蜗牛壳中化合物的检测结果。结果表明,在壳粉的其他成分中,有6种化合物占主导地位,分别是十六烷酸乙酯、(E)-9-十八烯酸乙酯、五烷酸、十七烷酸乙酯和9-十八烯酰胺。此外,该分析还分析了构成低百分比面积花园蜗牛壳结构的23多种化合物。这些物质分为脂肪酸、饱和脂肪酸、有机化合物、天然产物、甲酯和乙酯。因此,这些天然材料是必不可少的活性化合物,可以用于更多的医学和研究应用研究。但是,化合物的产率很低。综上所述,gc -质谱分析显示园螺壳中化学物质具有较高的生物多样性,可以帮助研究人员对园螺壳中不同的天然化学资源进行更多的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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