Investigation of physical, nutritional and sensory properties of wheat bread treated with purified thermostable cellulase and alpha amylase

Abstract

In this study, purified thermo- and acid-stable cellulase from the Bacillus licheniformis was used for improving physical and sensory properties of wheat bread. The thermostable cellulase was harvested, purified and characterized from isolated B. licheniformis species. The impact of parameters such as temperature, pH, and metal ions was tested on the purified cellulase enzyme. The purified enzyme was found to be stable at pH 4.0 and also thermally stable for 6 hat 45°C. Purified cellulase was used in preparation of wheat dough followed by the preparation of baked final product wheat bread. The final baked product showed a significant decrease in hardness, adhesiveness and also chewiness. The moisture-holding capacity was significantly increased compared to control. Furthermore, the effect of cellulase in combination with amylase was also studied in preparation of bread. The improvement in sensory parameters such as volume, color, texture, aroma and taste was observed on treating the dough with a combination of cellulase and amylase.