Study of Antihypertensive Activity from Red Quinoa Seed Protein Hydrolysate Digested by Various Protease Enzymes

Q3 Agricultural and Biological Sciences

HAYATI Journal of Biosciences Pub Date : 2023-11-01 DOI:10.4308/hjb.31.1.192-199

Dininurilmi Putri Suleman, None Harijono, Jue-Liang Hsu

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引用次数: 0

Abstract

Proteolytic enzymes are widely used to produce protein hydrolysates that contain bioactive peptides. Some of bioactive peptides are known inhibit the angiotensin-I-converting enzyme (ACE, EC 3.4.15.1) and act as human antihypertensive. Therefore, this study aims to produce protein hydrolysates via 16 hours of digestion process using Chenopodium formosanum (red quinoa) seed and the proteases, namely pepsin, trypsin, α-chymotrypsin, and thermolysin. The hydrolysates profiles and ACE-I inhibitory activity were analyzed using reversed phase-high performance liquid chromatography (RP-HPLC). The SDS-PAGE was also used to analyze the main storage protein in red quinoa seed, identified as being 11S seed storage globulin. Meanwhile, the ACE inhibitor activities of red quinoa seed protein (RQSP) produced by various proteases include the hydrolysate of pepsin 17.03% ± 3.88%, trypsin 42.67% ± 3.19%, α-chymotrypsin 72.71% ± 2.85% and thermolysin 77.67% ± 0.98%. These results show that red quinoa seed protein is a potential source of significant ACE inhibitor activity when hydrolyzed with α-chymotrypsin and thermolysin.

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不同蛋白酶水解红藜麦种子蛋白抗高血压活性的研究

蛋白水解酶被广泛用于生产含有生物活性肽的蛋白水解物。已知一些生物活性肽可抑制血管紧张素- i转换酶(ACE, EC 3.4.15.1)，具有抗高血压作用。因此，本研究旨在利用红藜麦种子和蛋白酶，即胃蛋白酶、胰蛋白酶、α-凝乳胰蛋白酶和热溶酶，经过16小时的消化过程，生产蛋白质水解产物。采用反相高效液相色谱法(RP-HPLC)分析其水解产物谱和ACE-I抑制活性。利用SDS-PAGE对红藜麦种子中的主要贮藏蛋白进行了分析，鉴定为11S贮藏球蛋白。红藜麦种子蛋白(RQSP)的ACE抑制剂活性分别为胃蛋白酶(17.03%±3.88%)、胰蛋白酶(42.67%±3.19%)、α-凝乳胰蛋白酶(72.71%±2.85%)和热溶酶(77.67%±0.98%)。这些结果表明，红藜麦种子蛋白与α-凝乳胰蛋白酶和热溶酶水解后可能具有显著的ACE抑制剂活性。

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来源期刊

HAYATI Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： HAYATI Journal of Biosciences (HAYATI J Biosci) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biosciences fields such as biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by HAYATI J Biosci. HAYATI J Biosci published by Department of Biology, Bogor Agricultural University, Indonesia and the Indonesian Society for Biology. We accept submission from all over the world. Our Editorial Board members are prominent and active international researchers in biosciences fields who ensure efficient, fair, and constructive peer-review process. All accepted articles will be published on payment of an article-processing charge, and will be freely available to all readers with worldwide visibility and coverage.