Analyzer-to-Analyzer Variations in Assaying Ultralow Concentrated Biomarkers Associated with Neurodegenerative Diseases Using Immunomagnetic Reduction

IF 4.6 Q1 CHEMISTRY, ANALYTICAL
Kun-Hung Lee, Ming-Hung Hsu, Hsin-Hsien Chen and Shieh-Yueh Yang*, 
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Abstract

By utilizing a high-temperature superconducting quantum interference device (high-Tc SQUID) magnetometer, an alternating current (AC) magnetosusceptometer, referred to as an analyzer, was developed for ultrasensitive immunoassays. The analyzer has been applied to assay biomarkers in human plasma associated with Alzheimer’s disease (AD) and Parkinson’s disease (PD). The involved assay methodology is the so-called immunomagnetic reduction (IMR). Such an analyzer has been approved for clinical use in Taiwan and Europe. The mass production of the analyzer is needed for clinical utilities. The issue of exploring analyzer-to-analyzer variations in the performances becomes critical. Unfortunately, there is no standard characterization to determine the variations in performances among analyzers. In this study, key characterizations, such as output signal stability, signal-to-noise ratio, measured concentrations of a control sample, etc., are proposed. In total, three analyzers are characterized in this work. The detected biomarkers include amyloid peptides, total tau protein, phosphorylated tau protein, and α-synuclein protein for AD and PD. Through one-way ANOVA for any of the characterizations among the three analyzers, it was found that there was no significant difference in any of these characterizations among the analyzers (p > 0.05). Furthermore, the three analyzers are applied to assay biomolecules for AD and PD in reference samples. High correlations (r > 0.8) in measured concentrations of any of these biomarkers in reference samples were obtained among the three analyzers. The results demonstrate that the proposed characterizations are feasible for achieving consistent performance among high-Tc SQUID-based AC magnetosusceptometers for assaying biomolecules.

Abstract Image

Abstract Image

利用免疫磁性还原法检测与神经退行性疾病相关的超低浓度生物标记物时分析仪之间的差异
通过利用高温超导量子干涉装置(high-Tc SQUID)磁力计,开发出了一种用于超灵敏免疫测定的交流(AC)磁感测器,称为分析仪。该分析仪已被用于检测人体血浆中与阿尔茨海默病(AD)和帕金森病(PD)相关的生物标记物。其中涉及的检测方法是所谓的免疫磁还原法(IMR)。这种分析仪已在台湾和欧洲获准用于临床。临床应用需要大量生产这种分析仪。探索分析仪之间的性能差异成为关键问题。遗憾的是,目前还没有标准的表征方法来确定分析仪之间的性能差异。在本研究中,我们提出了一些关键特性,如输出信号稳定性、信噪比、对照样本的测量浓度等。本研究共对三种分析仪进行了表征。检测的生物标记物包括淀粉样肽、总tau蛋白、磷酸化tau蛋白和α-突触核蛋白(AD和PD)。通过对三种分析仪的任何特征进行单因素方差分析,发现分析仪之间的任何特征均无显著差异(p > 0.05)。此外,这三种分析仪还被用于检测参考样本中的AD和PD生物大分子。三台分析仪在参考样本中测得的这些生物标记物的浓度具有很高的相关性(r > 0.8)。结果表明,所提出的特征描述是可行的,可以使基于高锝 SQUID 的交流磁差计在检测生物大分子时获得一致的性能。
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来源期刊
ACS Measurement Science Au
ACS Measurement Science Au 化学计量学-
CiteScore
5.20
自引率
0.00%
发文量
0
期刊介绍: ACS Measurement Science Au is an open access journal that publishes experimental computational or theoretical research in all areas of chemical measurement science. Short letters comprehensive articles reviews and perspectives are welcome on topics that report on any phase of analytical operations including sampling measurement and data analysis. This includes:Chemical Reactions and SelectivityChemometrics and Data ProcessingElectrochemistryElemental and Molecular CharacterizationImagingInstrumentationMass SpectrometryMicroscale and Nanoscale systemsOmics (Genomics Proteomics Metabonomics Metabolomics and Bioinformatics)Sensors and Sensing (Biosensors Chemical Sensors Gas Sensors Intracellular Sensors Single-Molecule Sensors Cell Chips Arrays Microfluidic Devices)SeparationsSpectroscopySurface analysisPapers dealing with established methods need to offer a significantly improved original application of the method.
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