The allelic spectrum of the GDF9/BSTHHI, CAST/MSPI, GH/HAEIII genes in Kal-myk short-tailed sheep and crossbreeds with a blood type of ? dorper + ? Kalmyk short-tailed breed

Abstract

To increase the production of meat productivity, it is necessary to improve the genetic resources of sheep. The purpose of this work was to establish the allelic spectrum of meat productivity genes in sheep of the Kalmyk Kalmyk short-tailed breed and crossbreeds with a blood type of ? dorper + ? Kalmyk short-tailed breed. The object of the study was sheep raised by the farm "Arl" of the Yashkul district of the Republic of Kalmykia. Blood samples (5 ml each) were taken from random sheep of the Kalmyk short-tailed breed (n = 14) and crossbreeds with a blood density of ? dorper + ? Kalmyk short-tailed breed (n = 12) from the jugular vein into a test tube with ethylenediaminetetraacetic acid (EDTA). The PCR-PDRF method determined the allelic spectrum of genes: differential growth factor (GDF9), calpastatin (CAST), somatotropin (growth hormone (GH)), in Kalmyk sheepdogs and crossbreeds with a blood type of ? dorper + Kalmyk short-tailed breed. The methods of genetic and statistical analysis in the studied samples of sheep revealed complex genotypes for the studied marker genes. By genetic and statistical methods of analysis, it was found that the proportion of animals that had the desired complex genotype according to the studied genes in sheep of the Kalmyk chicken breed, including – 1 marker allele in three genes (GDF9AG/CTMM/GHAA and GDF9GG/CASTMN/GHAA), was 28.6%, in turn, in crossbreeds with a blood type of ? dorper + A combination of 2 marker alleles of three genes (GDF9AG/CASTMN/GHAA) appeared in the Kalmyk short-tailed breed - 33.3%, a combination of the 1st marker allele in three genes - 50.0%. It is concluded that the crossing of Kalmyk sheep of the Kurdyuk breed with sheep-producers of the Dorper breed, carrying alleles desirable for breeding in their genome, can have a positive effect on increasing the productivity of crossbreed livestock.