Studies on the extraction performance of phorate by aptamer-functionalized magnetic nanoparticles in plasma samples

IF 3.8 4区工程技术 Q2 CHEMISTRY, MULTIDISCIPLINARY

Green Processing and Synthesis Pub Date : 2023-01-01 DOI:10.1515/gps-2023-0065

Ting Wang, Junpeng Tan, Shenghui Xu, Yong Li, Hongxia Hao

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引用次数: 0

Abstract

Abstract Phorate, a highly toxic organophosphorus pesticide, poses significant risks due to its efficiency, versatility, and affordability. Therefore, studying pretreatment and detection methods for phorate in complex samples is crucial. In this study, we synthesized core-shell phorate aptamer-functionalized magnetic nanoparticles using solvothermal and self-assembly techniques. Subsequently, we developed a magnetic dispersive solid-phase extraction and detection method to identifying phorate in plasma samples. Under optimal conditions, we achieved quantitation of phorate within a range of 2–700 ng·mL −1 using gas chromatography-mass spectrometry. The detection limit ( S / N = 3) was 0.46 ng·mL −1 , and the intraday and interday relative standard deviation were 3.4% and 4.1%, respectively. In addition, the material exhibited excellent specificity, an enrichment capacity (EF = 416), and reusability (≥15). During phorate extraction from real plasma samples, spiked recoveries ranged from 86.1% to 101.7%. These results demonstrate that our method offers superior extraction efficiency and detection capability for phorate in plasma samples.

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适体功能化磁性纳米颗粒在等离子体样品中提取磷酸盐性能的研究

摘要膦酸盐是一种剧毒有机磷农药，由于其高效、多用途和可负担性，造成了巨大的风险。因此，研究复杂样品中磷酸盐的预处理和检测方法至关重要。在这项研究中，我们利用溶剂热和自组装技术合成了核-壳磷酸适配体功能化的磁性纳米颗粒。随后，我们开发了一种磁色散固相萃取检测方法来鉴定血浆样品中的磷酸盐。在最佳条件下，我们使用气相色谱-质谱法在2-700 ng·mL−1范围内实现了磷酸盐的定量。检出限(S / N = 3)为0.46 ng·mL−1，日内和日间相对标准偏差分别为3.4%和4.1%。此外，该材料还具有良好的特异性、富集能力(EF = 416)和可重复使用性(≥15)。在实际血浆样品中提取磷酸盐时，加标回收率为86.1% ~ 101.7%。这些结果表明，我们的方法对血浆样品中的磷酸盐具有较高的提取效率和检测能力。

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来源期刊

Green Processing and Synthesis CHEMISTRY, MULTIDISCIPLINARY-ENGINEERING, CHEMICAL

CiteScore

6.70

自引率

9.30%

发文量

审稿时长

7 weeks

期刊介绍： Green Processing and Synthesis is a bimonthly, peer-reviewed journal that provides up-to-date research both on fundamental as well as applied aspects of innovative green process development and chemical synthesis, giving an appropriate share to industrial views. The contributions are cutting edge, high-impact, authoritative, and provide both pros and cons of potential technologies. Green Processing and Synthesis provides a platform for scientists and engineers, especially chemists and chemical engineers, but is also open for interdisciplinary research from other areas such as physics, materials science, or catalysis.