Study on spatio-temporal prevalence and hematological attributes of bovine Babesiosis in cattle population of Layyah, Southern Punjab, Pakistan

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia Pub Date : 2023-10-01 DOI:10.1590/1678-4162-13002

A. Ahmad, Z. Ali, M.H. Lashari

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Abstract

ABSTRACT The present study was conducted to investigate the spatiotemporal prevalence of bovine babesiosis in cattle population (n=376) of Layyah, Pakistan as affected by location, age, breed, gender, and seasons. Blood samples were collected aseptically and assessed for babesiosis through microscopy and PCR, and through automated analyzer for hematological attributes. Overall results of prevalence through PCR in cattle population showed significantly (P≤0.05) lower prevalence of 19.4% (n=72/376) as compared to 37.2% (n=140/276) through microscopy. None of the studied cattle from Cholistani breed were Babesia-positive. However, significantly (P≤0.05) higher prevalence was noticed for crossbred cattle (46.7%, n= 50/107) followed by that in Friesian (16.1%, n= 10/62), Jersey (7%, n= 5/71) and Sahiwal (6.9%, n= 7/101) cattle breeds. Female cattle (19.5%, n= 55/281) and age group 1 (Up to 2 years) (40%, n= 42/105) had higher prevalence of Babesia as ascertained through PCR in comparison to their counterpart groups. Significantly (P≤0.05) higher prevalence of 35.9% (n=60/167) was shown in summer as compared to that in winter season (5.7%, n= 12/209). All the positive samples produced the 490bp amplicons specific and typical for Babesia bigemina. Hemoglobin concentration, erythrocytic count, hematocrit and mean corpuscular volume were significantly (P≤0.05) lower in babesia-positive cattle as compared to healthy ones.

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巴基斯坦旁遮普省南部Layyah地区牛巴贝斯虫病时空流行及血液学特征研究

摘要本研究调查了巴基斯坦Layyah牛种群(n=376)中牛巴贝斯虫病的时空流行情况，以及地点、年龄、品种、性别和季节对牛巴贝斯虫病的影响。无菌采集血样，通过显微镜和PCR检测巴贝斯虫病，并通过自动血液学分析仪检测血液特性。通过PCR在牛群中的总体患病率结果显示，与显微镜下的37.2% (n=140/276)相比，前者的患病率为19.4% (n=72/376)，显著(P≤0.05)降低。所研究的牛品种中没有巴贝斯虫阳性。其中，杂交牛(46.7%，n= 50/107)的患病率最高(P≤0.05)，其次是弗里西亚(16.1%，n= 10/62)、泽西(7%，n= 5/71)和萨希瓦尔(6.9%，n= 7/101)。通过PCR确定，母牛(19.5%，n= 55/281)和年龄1(2岁以下)(40%，n= 42/105)的巴贝斯虫患病率高于其对应组。夏季患病率为35.9% (n=60/167)，高于冬季(5.7%，n= 12/209)，差异有统计学意义(P≤0.05)。所有阳性样本均产生双巴贝斯虫特异性和典型的490bp扩增子。与健康牛相比，巴贝虫病阳性牛血红蛋白浓度、红细胞计数、红细胞压积和平均红细胞体积显著(P≤0.05)降低。

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来源期刊

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia 农林科学-兽医学

CiteScore

0.80

自引率

25.00%

发文量

111

审稿时长

9-18 weeks

期刊介绍： Publica artigos originais de pesquisa sobre temas de medicina veterinária, zootecnia, tecnologia e inspeção de produtos de origem animal e áreas afins relacionadas com a produção animal. Atualmente a revista mantém 628 permutas (419 internacionais e 209 nacionais), sendo um verdadeiro suporte para o recebimento de periódicos pela Biblioteca da Escola. A partir de 1999, a Escola de Veterinária delegou à FEP MVZ Editora o encargo do gerenciamento e edição de todas suas publicações, inclusive do Arquivo, ficando somente com o apoio logístico (instalações, equipamentos, pessoal etc.). O apoio financeiro é exercido pelo CNPq/FINEP e pela própria FEP MVZ.