Can bovine embryos be successfully transferred after 40 years of cryopreservation?

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia Pub Date : 2023-10-01 DOI:10.1590/1678-4162-13035

J. Camacho-Ronquillo, E. Posadas-Manzano, F. Utrera-Quintana, J. Hernández-Hernández, J. Gallegos-Sánchez, J. Cruz-Aviña, A. Villa-Mancera

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Abstract

ABSTRACT Global advances in reproductive biotechnology have allowed for the transfer of embryos from donor females with high genetic merit to recipients using the cryopreservation technique, which preserves an embryo of excellent quality and viability, thereby achieving a feasible pregnancy rate. The objective of this study was to evaluate the quality and viability of Holstein embryos that have been cryopreserved for more than 40 years under glycerol freezing. The embryos were transferred to the recipient heifers using a non-surgical method. Two 17-month-old Holstein heifers (360 kg live weights) which were clinically healthy and reproductively active were used as the recipients. Two bovine embryos of Grade 1 quality were thawed and evaluated for their morphology. Of the two embryo transfers, one pregnancy was achieved, resulting in the birth of a calf. Therefore, embryos frozen in liquid nitrogen and glycerol as a cryopreservative for more than 40 years maintained their quality and viability to produce a live calf.

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牛胚胎冷冻保存40年后能否成功移植?

生殖生物技术的全球进步已经允许使用冷冻保存技术将具有高遗传价值的雌性供体胚胎移植到受体，这种技术保存了高质量和活力的胚胎，从而实现了可行的妊娠率。本研究的目的是评估在甘油冷冻下冷冻保存超过40年的荷斯坦胚胎的质量和活力。胚胎通过非手术方法移植到受体小牛身上。2头临床健康且繁殖活跃的17月龄荷斯坦小母牛(活重360 kg)作为受体。对2个质量为1级的牛胚胎进行解冻和形态学评价。在两次胚胎移植中，有一次成功怀孕，并生下了一头小牛。因此，胚胎在液氮和甘油中冷冻40多年，以保持其质量和生产活牛犊的活力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia 农林科学-兽医学

CiteScore

0.80

自引率

25.00%

发文量

111

审稿时长

9-18 weeks

期刊介绍： Publica artigos originais de pesquisa sobre temas de medicina veterinária, zootecnia, tecnologia e inspeção de produtos de origem animal e áreas afins relacionadas com a produção animal. Atualmente a revista mantém 628 permutas (419 internacionais e 209 nacionais), sendo um verdadeiro suporte para o recebimento de periódicos pela Biblioteca da Escola. A partir de 1999, a Escola de Veterinária delegou à FEP MVZ Editora o encargo do gerenciamento e edição de todas suas publicações, inclusive do Arquivo, ficando somente com o apoio logístico (instalações, equipamentos, pessoal etc.). O apoio financeiro é exercido pelo CNPq/FINEP e pela própria FEP MVZ.