Extract and fraction of cashew nut testa ameliorate the hyperglycemic mice induced by Streptozotocin and high-fat diet

IF 0.7 Q4 PLANT SCIENCES
Huy Duc Van, Ly Thi Thao Nguyen, Nhan Lu Chinh Phan, Thuy Thi Thanh Dao, Long Thanh Le, Nhu Thi Ngoc Nguyen, Huynh Ton Ngoc Nguyen, Loan Thi Tung Dang
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Abstract

Drug strategy is a standard method for treating type 2 diabetes mellitus (T2D), a non-communicable disease with increasing prevalence, which may cause side effects. Therefore, natural compounds with limited adverse effects have come back into vogue for treating T2D. This study aims to evaluate the effects on rehabilitating hyperglycemic mice of cashew nut testa (husk) extract and fraction known as potential bio-substances for improvement in T2D. First, the hyperglycemic mice were induced with a high-fat diet (HFD) for 4 weeks and then were injected with streptozotocin (STZ, dozen for injection was 40 mg/kg/week) for 2 weeks. Next, the confirmed hyperglycemic mice were treated with pioglitazone (HG+PG group), total extract (HG+TE group), and saponin-rich fraction (HG+SRF group) for 3 weeks. Then, the evaluation was based on body mass; blood glucose (BG) level; BG tolerance, lipid profile, pancreatic histology and the expression IRS-1 in the pancreas. The results showed that body mass and BG level significantly increased in hyperglycemic mice. After substance treatment, there was no change in body mass in TE and SRF groups. However, BG level of HG+TE group mice significantly decreased compared to hyperglycemic mice and only BG tolerance of HG+SRF group was improved. Besides, HG+TE and HG+SRF groups modulated the triglyceride, HDL and LDL close to those expressed in normal mice. In addition, histological images of the pancreas revealed the restoration in both HG+TE and HG+SRF groups. Simultaneously, the IRS-1 expression in HG+TE group pancreas was restored to its expression in normal mice. These results demonstrate that the TE and SRF of cashew nut testa could ameliorate BG, lipid profile and pancreatic IRS-1 expression and restore the damaged pancreas and islets in hyperglycemic mice.
腰果提取物及提取物对链脲佐菌素和高脂饮食诱导的高血糖小鼠有改善作用
药物策略是治疗2型糖尿病(T2D)的标准方法,这是一种发病率越来越高的非传染性疾病,可能会产生副作用。因此,副作用有限的天然化合物已重新成为治疗T2D的时尚。本研究旨在评价腰果皮提取物及其提取物对高血糖小鼠的修复作用,腰果皮提取物和提取物是改善t2dm的潜在生物物质。先用高脂饮食(HFD)诱导高血糖小鼠4周,然后注射链脲佐菌素(STZ,注射剂量为40 mg/kg/周)2周。随后,确诊的高血糖小鼠分别给予吡格列酮(HG+PG组)、总提取物(HG+TE组)、富皂苷部分(HG+SRF组)治疗3周。然后,以体重为评价标准;血糖水平;BG耐量,血脂,胰腺组织学和IRS-1在胰腺中的表达。结果表明,高血糖小鼠的体重和血糖水平明显升高。药物治疗后,TE组和SRF组的体重没有变化。与高血糖小鼠相比,HG+TE组小鼠的BG水平明显降低,仅HG+SRF组小鼠的BG耐量有所提高。HG+TE和HG+SRF组对甘油三酯、HDL和LDL的调节水平与正常小鼠相近。此外,胰腺组织学图像显示HG+TE组和HG+SRF组均有恢复。同时,HG+TE组胰腺中IRS-1的表达恢复到正常小鼠的水平。上述结果表明,腰果tef和腰果SRF可以改善高血糖小鼠的BG、血脂和胰腺IRS-1表达,恢复受损的胰腺和胰岛。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant Science Today
Plant Science Today PLANT SCIENCES-
CiteScore
1.50
自引率
11.10%
发文量
177
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