Robust Porcine GFR Measurement with Radiotracers and Only Late Blood Samples

Lars Jødal, Juan Ignacio Brignone, Pui-Ki Chan Ladefoged, Lars Lund, Trine Borup Andersen
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Abstract

(1) Pigs are physiologically very relevant as animal models of human physiology. Radiotracer methods for porcine GFR (glomerular filtration rate) determination exist but require full-curve blood sampling or the application of correction formulas, which vary among studies. (2) We used porcine GFR data (40 datapoints from 20 juvenile pigs) for which the GFR was measured as the plasma clearance of [99mTc]Tc-DTPA. The reference clearance (Cl, GFR; range 41–85 mL/min) was measured from the full curve under the data. For simpler determination, an approximate clearance, Cl1, was based on the last five blood samples (acquired 120–240 min post injection). (3) The following formula for the GFR was developed: Cl = 1.27 · (Cl1)0.92. The spread (SD) was within 4% of the reference GFR. A comparison with the literature data showed that our correction formula was robust in pigs of various breeds, sizes up to approximately 200 kg, and GFRs up to approximately 400 mL/min, with a spread of up to 8%. The formula was also applicable for iohexol as the tracer. (4) A formula was developed that allows porcine GFR to be measured using only 4–5 late blood samples. This new formula can be applied across a wide range of swine breeds, animal sizes, and GFR ranges, allowing for robust determination of the GFR in pigs without full-curve blood sampling and without urine collection.
用放射性示踪剂和仅晚期血液样本测定猪GFR
(1)猪作为人类生理的动物模型在生理学上是非常相关的。目前存在测定猪肾小球滤过率的放射性示踪剂方法,但需要全曲线采血或应用校正公式,这在不同的研究中有所不同。(2)我们使用猪GFR数据(来自20头仔猪的40个数据点),GFR作为血浆中[99mTc]Tc-DTPA的清除率。参考间隙(Cl, GFR;范围41-85 mL/min)从数据下的全曲线测量。为了更简单的测定,根据注射后120-240分钟采集的最后5份血液样本,获得近似的清除率Cl1。(3)得到GFR的计算公式:Cl = 1.27·(Cl1)0.92。差值(SD)在参考GFR的4%以内。与文献数据的比较表明,我们的修正公式适用于各种品种的猪,体型约为200公斤,gfr约为400 mL/min,传播率可达8%。该公式同样适用于碘己醇作为示踪剂。(4)开发了一种公式,可以仅使用4 - 5份晚期血液样本来测量猪GFR。该新公式可广泛应用于猪品种、动物大小和GFR范围,无需全曲线血液采样和尿液收集即可可靠地测定猪的GFR。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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