Orthogonal Surface-Enhanced Raman Scattering/Field-Effect Transistor Detection of Breast and Colorectal Cancer-Derived Exosomes using Graphene as a Tag-Free Diagnostic Template

IF 4 Q2 ENGINEERING, BIOMEDICAL

Advanced Nanobiomed Research Pub Date : 2023-10-08 DOI:10.1002/anbr.202300055

Bruno Gil, Meysam Keshavarz, Dominic Wales, Ara Darzi, Eric Yeatman

{"title":"Orthogonal Surface-Enhanced Raman Scattering/Field-Effect Transistor Detection of Breast and Colorectal Cancer-Derived Exosomes using Graphene as a Tag-Free Diagnostic Template","authors":"Bruno Gil, Meysam Keshavarz, Dominic Wales, Ara Darzi, Eric Yeatman","doi":"10.1002/anbr.202300055","DOIUrl":null,"url":null,"abstract":"<p>Cancer is one of the leading causes of high mortality worldwide, affecting most of the body organs including the breast and colon. Triple-negative breast (TNBC) and colorectal cancers (CRC) usually present the poorest clinical outcomes and lowest disease-free survival rates among the different cancer types. Novel biomarkers for early detection of TNBC and CRC have been proposed throughout the years, although the rate of success remains limited. Herein, a dual method for detection of cancer-derived exosomes based on the measurement of the Raman spectra in graphene field-effect transistors (GFETs) is proposed, thereby obtaining electrical signal metrics related to the variation of the Dirac point in graphene and optical features corresponding to representative bio-molecular cancer structures in an orthogonal way. This pioneering method and classification routine can potentially be extended to other types of cancer, thus creating a unique and universal tag-free diagnostic template for early cancer diagnostics.</p>","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"3 11","pages":""},"PeriodicalIF":4.0000,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300055","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Nanobiomed Research","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/anbr.202300055","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Cancer is one of the leading causes of high mortality worldwide, affecting most of the body organs including the breast and colon. Triple-negative breast (TNBC) and colorectal cancers (CRC) usually present the poorest clinical outcomes and lowest disease-free survival rates among the different cancer types. Novel biomarkers for early detection of TNBC and CRC have been proposed throughout the years, although the rate of success remains limited. Herein, a dual method for detection of cancer-derived exosomes based on the measurement of the Raman spectra in graphene field-effect transistors (GFETs) is proposed, thereby obtaining electrical signal metrics related to the variation of the Dirac point in graphene and optical features corresponding to representative bio-molecular cancer structures in an orthogonal way. This pioneering method and classification routine can potentially be extended to other types of cancer, thus creating a unique and universal tag-free diagnostic template for early cancer diagnostics.

Abstract Image

查看原文本刊更多论文

使用石墨烯作为无标签诊断模板的正交表面增强拉曼散射/场效应晶体管检测乳腺癌和结直肠癌衍生外泌体

癌症是全球高死亡率的主要原因之一，影响包括乳房和结肠在内的大多数身体器官。在不同类型的癌症中，三阴性乳腺癌(TNBC)和结直肠癌(CRC)通常表现出最差的临床结果和最低的无病生存率。近年来，人们提出了用于早期检测TNBC和CRC的新型生物标志物，尽管成功率仍然有限。本文提出了一种基于石墨烯场效应晶体管(gfet)中拉曼光谱测量的双重检测癌症衍生外体的方法，从而以正交的方式获得与石墨烯中狄拉克点变化相关的电信号度量和与代表性生物分子癌症结构对应的光学特征。这种开创性的方法和分类程序可以潜在地扩展到其他类型的癌症，从而为早期癌症诊断创建一个独特和通用的无标签诊断模板。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Advanced Nanobiomed Research nanomedicine, bioengineering and biomaterials-

CiteScore

5.00

自引率

5.90%

发文量

审稿时长

21 weeks

期刊介绍： Advanced NanoBiomed Research will provide an Open Access home for cutting-edge nanomedicine, bioengineering and biomaterials research aimed at improving human health. The journal will capture a broad spectrum of research from increasingly multi- and interdisciplinary fields of the traditional areas of biomedicine, bioengineering and health-related materials science as well as precision and personalized medicine, drug delivery, and artificial intelligence-driven health science. The scope of Advanced NanoBiomed Research will cover the following key subject areas: ▪ Nanomedicine and nanotechnology, with applications in drug and gene delivery, diagnostics, theranostics, photothermal and photodynamic therapy and multimodal imaging. ▪ Biomaterials, including hydrogels, 2D materials, biopolymers, composites, biodegradable materials, biohybrids and biomimetics (such as artificial cells, exosomes and extracellular vesicles), as well as all organic and inorganic materials for biomedical applications. ▪ Biointerfaces, such as anti-microbial surfaces and coatings, as well as interfaces for cellular engineering, immunoengineering and 3D cell culture. ▪ Biofabrication including (bio)inks and technologies, towards generation of functional tissues and organs. ▪ Tissue engineering and regenerative medicine, including scaffolds and scaffold-free approaches, for bone, ligament, muscle, skin, neural, cardiac tissue engineering and tissue vascularization. ▪ Devices for healthcare applications, disease modelling and treatment, such as diagnostics, lab-on-a-chip, organs-on-a-chip, bioMEMS, bioelectronics, wearables, actuators, soft robotics, and intelligent drug delivery systems. with a strong focus on applications of these fields, from bench-to-bedside, for treatment of all diseases and disorders, such as infectious, autoimmune, cardiovascular and metabolic diseases, neurological disorders and cancer; including pharmacology and toxicology studies.