Burden of Rotavirus (RV) Infection in Children Below Five Years of Age Having Acute Diarrhoea and Pattern of Circulating Genotypes in a Community in Indore City of Madhya Pradesh

Abstract

Background: Determining the prevalence of rotavirus in a specificarea can provide valuable insights regarding its seasonality, aid thehealthcare system in effectively managing cases of acute diarrhoea,and contribute to the prevention of drug misuse.Aim: To study the burden of rotavirus infection in children below fiveyears of age having acute diarrhoea and pattern of circulating genotypesin a community in Indore city of Madhya Pradesh.Methods: Children below five years of age with acute diarrhoea provideda total of 100 stool samples, which were then tested for the presenceof rotavirus antigen using an enzyme immunoassay (EIA). After that,RT-PCR was used to genotype the EIA-positive samples’ G and P types.Demographic and clinical information were recorded on a proforma.Results: The prevalence of rotavirus infection in children below fiveyears of age having acute diarrhoea was 17%. Nine typeable rotavirusstrains out of a total of 17 were typed using RT-PCR. One strain (11.1%)was partially typed, two strains (22.2%) were not typeable, and sixstrains (66.7%) were fully genotyped for the G and P genotypes. TheG1 genotype was found in the greatest number of strains (5, 71.4%),followed by G2 and G10 in 1 strain each (14.3% each). P8 and P4 werefound in one strain each (50.0% each).Conclusion: The majority of rotavirus strains were G1 genotyped. Inorder to boost the regional specificity of multivalent RV vaccines, thisstudy emphasises the need to include new rotavirus strains. How to cite this article:Gupta A, Shah H. Burden of Rotavirus (RV)Infection in Children Below Five Years of AgeHaving Acute Diarrhoea and Pattern of CirculatingGenotypes in a Community in Indore City ofMadhya Pradesh. Chettinad Health City Med J.2023;12(3):41-44. DOI: https://doi.org/10.24321/2278.2044.202349