Dielectrophoretic Movement of Cell Passing Between Surface Electrodes in Flow Channel

Shigehiro Hashimoto, Ryuya Ono
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Abstract

Abstract In this study, cell behavior in a microchannel was tracked for the application of dielectrophoresis to biological cell sorting. A pair of titanium surface electrodes was fabricated inside the microchannel by photolithographic techniques: a triangular electrode with a tip angle of 0.26 rad and a rectangular electrode. A periodic alternating current of square wave with a period of 1 μs was introduced between the electrodes to induce an asymmetric electric field perpendicular to the mainstream direction. The behavior of mouse myoblasts (C2C12: mouse myoblast cell line) was measured in vitro while the suspension was flowing. The relationship between cell shift motion near the electrode and cell shape on the two-dimensional projection plane was investigated. Experimental results showed that cell movement in the direction perpendicular to the mainstream increased with geometries away from the circle in the two-dimensional projection plane. This method can be applied to sort cells according to their degree of shape deviation from a sphere. The dielectrophoretic effect can be applied to sort cells not only by cell size but also by cell deformation.
流动通道中细胞在表面电极间传递的介电泳运动
摘要本研究对微通道中的细胞行为进行了跟踪,以便将介质电泳技术应用于生物细胞分选。采用光刻技术在微通道内制备了一对钛表面电极:一个尖端角为0.26 rad的三角形电极和一个矩形电极。在电极间引入周期为1 μs的方波交流电,产生垂直于主流方向的不对称电场。小鼠成肌细胞(C2C12:小鼠成肌细胞系)在体外流动时的行为。研究了细胞在电极附近的移动运动与细胞在二维投影平面上的形状之间的关系。实验结果表明,在二维投影平面上,当几何形状远离圆时,细胞在垂直于主流方向上的运动增加。这种方法可以根据细胞的形状偏离球体的程度来进行分类。介电泳效应不仅可以应用于细胞大小,还可以应用于细胞变形。
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