OsBLS6.2: A rice bacterial leaf streak resistance gene identified by GWAS and RNA-seq

IF 6 1区农林科学 Q1 AGRONOMY

Crop Journal Pub Date : 2023-12-01 DOI:10.1016/j.cj.2023.08.007

Huabin Xie , Chunzi Lin , Wenyu Lu , Zhikai Han , Danhong Wei , Xing Huo , Tianjiao Li , Jian Zhang , Yongqiang He , Chun Chen , Hui Wang , Tao Guo , Jiafeng Wang

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引用次数: 0

Abstract

Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzicola (Xoc), is a bacterial disease affecting rice production in Asia and Africa, whose severity is expected to increase with climate change. Identification of new quantitative-trait loci (QTL) or resistance genes for BLS resistance is essential for developing resistant rice. A genome-wide association study to identify QTL associated with BLS resistance was conducted using phenotypic and genotypic data from 429 rice accessions. Of 47 QTL identified, 45 were novel and two co-localized with previously reported QTL or genes conferring BLS resistance. qBLS6.2 on chromosome 6 explained the greatest phenotypic variation. Combined analysis of differential expression and annotations of predicted genes near qBLS6.2 based on haplotype and disease phenotype identified OsBLS6.2 (LOC_Os06g02960) as a candidate gene for qBLS6.2. OsBLS6.2 knockout plants showed higher resistance to Xoc than wild-type plants. Many other candidate genes for resistance to Xoc were identified.

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OsBLS6.2：通过 GWAS 和 RNA-seq 鉴定的水稻细菌性叶斑病抗性基因

由黄单胞菌（Xanthomonas oryzae pv. oryzicola，Xoc）引起的细菌性叶斑病（BLS）是影响亚洲和非洲水稻生产的一种细菌性病害，其严重程度预计将随着气候变化而加剧。鉴定抗BLS的新数量性状位点（QTL）或抗性基因对于开发抗性水稻至关重要。利用 429 个水稻品种的表型和基因型数据，开展了一项全基因组关联研究，以鉴定与 BLS 抗性相关的 QTL。在鉴定出的 47 个 QTL 中，45 个是新的，2 个与之前报道的 QTL 或赋予 BLS 抗性的基因共定位。根据单倍型和疾病表型对 qBLS6.2 附近的预测基因的差异表达和注释进行了综合分析，确定 OsBLS6.2 (LOC_Os06g02960) 为 qBLS6.2 的候选基因。OsBLS6.2 基因敲除植株对 Xoc 的抗性高于野生型植株。研究还发现了许多其他抗 Xoc 的候选基因。

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来源期刊

Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

9.90

自引率

3.00%

发文量

638

审稿时长

41 days

期刊介绍： The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.