The ZooScan and the ZooCAM zooplankton imaging systems are intercomparable: A benchmark on the Bay of Biscay zooplankton

IF 2.1 3区 地球科学 Q2 LIMNOLOGY
Nina Grandremy, Christine Dupuy, Pierre Petitgas, Sophie Le Mestre, Paul Bourriau, Antoine Nowaczyk, Bertrand Forest, Jean-Baptiste Romagnan
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Abstract

Zooplankton analysis represents a bottleneck in marine ecology studies due to the difficulty to obtain zooplankton data. The last decades have seen the intense development of zooplankton imaging systems, to increase the zooplankton data spatio-temporal resolution as well as enabling the combination of size, taxonomy, and functional traits in aquatic ecology studies. Here, we propose a benchmark between the ZooScan, a commercially available, laboratory-based scanner, which analyses zooplankton preserved samples, and the ZooCAM, an in-flow imaging system designed for on-board live zooplankton imaging. Sixty-one zooplankton samples collected over the Bay of Biscay in environments ranging from estuarine to offshore blue waters were imaged with both instruments. Zooplankton Normalized Biovolume-Size Spectra slopes, mean sizes, abundances, and zooplankton community biogeographical patterns were computed for each instrument and compared at the taxonomic group, the sampling stations and the Bay of Biscay scales. Both instruments produced similar zooplankton variables by stations and by taxa and described similar zooplankton community compositions and biogeographical patterns, on the large mesozooplankton size range, i.e., [0.3–3.39] mm ESD. We conclude that the ZooCAM and the ZooScan data can be combined to generate long term or spatially resolved zooplankton time series. Our study shows that benchmarking imaging instruments or techniques (1) offers a robust assessment of interoperability between instruments, mitigating possible instrumental biases, and (2) may be of great interest in the case of instrumental obsolescence or breakdown, to choose the most conservative replacement solution in a long term time series framework.

Abstract Image

ZooScan和ZooCAM浮游动物成像系统具有可比性:比斯开湾浮游动物的基准
由于浮游动物数据难以获取,浮游动物分析成为海洋生态学研究的瓶颈。近几十年来,浮游动物成像系统得到了大力发展,以提高浮游动物数据的时空分辨率,并使水生生态学研究中浮游动物的大小、分类和功能特征相结合。在这里,我们提出了ZooScan和ZooCAM之间的基准,ZooScan是一种商用的、基于实验室的扫描仪,用于分析浮游动物保存的样本,ZooCAM是一种为船上活体浮游动物成像而设计的流内成像系统。用这两种仪器对比斯开湾从河口到近海蓝色水域的环境中收集的61种浮游动物样本进行了成像。计算了每种仪器的归一化生物体积-大小光谱斜率、平均大小、丰度和浮游动物群落生物地理格局,并在分类群、采样站和比斯开湾尺度上进行了比较。在较大的中浮游动物尺寸范围(即[0.3-3.39]mm ESD)上,这两种工具都产生了相似的浮游动物站和分类群变量,并描述了相似的浮游动物群落组成和生物地理格局。我们得出结论,ZooCAM和ZooScan数据可以结合起来产生长期或空间分辨的浮游动物时间序列。我们的研究表明,对成像仪器或技术进行基准测试(1)提供了对仪器之间互操作性的可靠评估,减轻了可能的仪器偏差;(2)在仪器过时或故障的情况下,在长期时间序列框架中选择最保守的替代解决方案可能非常有趣。
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来源期刊
CiteScore
4.80
自引率
3.70%
发文量
56
审稿时长
3 months
期刊介绍: Limnology and Oceanography: Methods (ISSN 1541-5856) is a companion to ASLO''s top-rated journal Limnology and Oceanography, and articles are held to the same high standards. In order to provide the most rapid publication consistent with high standards, Limnology and Oceanography: Methods appears in electronic format only, and the entire submission and review system is online. Articles are posted as soon as they are accepted and formatted for publication. Limnology and Oceanography: Methods will consider manuscripts whose primary focus is methodological, and that deal with problems in the aquatic sciences. Manuscripts may present new measurement equipment, techniques for analyzing observations or samples, methods for understanding and interpreting information, analyses of metadata to examine the effectiveness of approaches, invited and contributed reviews and syntheses, and techniques for communicating and teaching in the aquatic sciences.
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