Lab on a chip for detecting Clara cell protein 16 (CC16) for potential screening of the workers exposed to respirable silica aerosol

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Chong Ahn, Taekhee Lee, Jae Hoon Shin, Jong Seong Lee, V. Thiyagarajan Upaassana, Sthitodhi Ghosh, Bon Ki Ku
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Abstract

Early detection of pulmonary responses to silica aerosol exposure, such as lung inflammation as well as early identification of silicosis initiation, is of great importance in disease prevention of workers. In this study, to early screen the health condition of the workers who are exposed to respirable silica dusts, an immunoassay lab on a chip (LOC) was designed, developed and fully characterized for analyzing Clara cell protein 16 (CC16) in serum which has been considered as one of the potential biomarkers of lung inflammation or lung damage due to the respirable silica dusts. Sandwich immunoassay of CC16 was performed on the LOC developed with a custom-designed portable analyzer using artificial serums spiked with CC16 protein first and then human serums obtained from the coal mine workers exposed to the respirable silica-containing dusts. The dynamic range of CC16 assay performed on the LOC was in a range of 0.625–20 ng/mL, and the achieved limit of detection (LOD) was around 0.35 ng/mL. The assay results of CC16 achieved from both the developed LOC and the conventional 96 well plate showed a reasonable corelation. The correlation between the conventional reader and the developed portable analyzer was found to be reasonable, resulting in R2 ~ 0.93. This study shows that the LOC developed for the early detection of CC16 can be potentially applied for the development of a field-deployable point-of-care testing (POCT) for the early monitoring of the field workers who are exposed to silica aerosol.

Abstract Image

实验室芯片检测克拉拉细胞蛋白16 (CC16)的潜在筛选暴露于可吸入二氧化硅气溶胶的工人
早期发现肺部对二氧化硅气溶胶暴露的反应,如肺部炎症以及早期识别矽肺病的发病,对工人的疾病预防具有重要意义。为了早期筛查暴露于可吸入二氧化硅粉尘的工人的健康状况,本研究设计、开发了一种免疫分析芯片实验室(LOC),用于分析血清中被认为是可吸入二氧化硅粉尘引起肺部炎症或肺损伤的潜在生物标志物之一的Clara细胞蛋白16 (CC16)。CC16的夹心免疫测定采用定制的便携式分析仪,首先使用添加CC16蛋白的人工血清,然后使用从暴露于可呼吸性含二氧化硅粉尘的煤矿工人中提取的人血清。CC16测定的LOC动态范围为0.625 ~ 20 ng/mL,检出限(LOD)约为0.35 ng/mL。CC16的测定结果与常规96孔板具有较好的相关性。发现传统阅读器与研制的便携式分析仪的相关性合理,R2 ~ 0.93。这项研究表明,为早期检测CC16而开发的LOC可以潜在地应用于开发现场可部署的护理点测试(POCT),用于早期监测暴露于二氧化硅气溶胶的现场工作人员。
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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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