Evaluation of Root and Leaf Extracts of Glycrriza Glabra for Antimicrobial Activity

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI:10.12720/jomb.4.1.81-85

H. Aggarwal, Jayashri Ghosh, A. Rao, V. Chhokar

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引用次数: 8

Abstract

Glycyrrhiza glabra is a traditional herb which grows in various parts of the world. It is a very sweet, moist, soothing herb that detoxifies and protects the liver. It also has a powerful anti-inflammatory action and also finds applications in arthritis and mouth ulcers. In the present study, antibacterial activity of Glycyrrhiza glabra root and leaf extracts were tested against Escherichia coli, Pseudomonas aeruginosa, Entereobacter cloacae and Klebsiella sp. using agar well/disc diffusion assay. The extracts obtained by cold maceration method and Soxhlet method could not inhibit the test bacteria. Both the leaf and root extracts prepared using reflux extraction method exhibited strong antibacterial activity against Escherichia coli and Pseudomonas aeruginosa. However these were found to be ineffective against Enterobacter cloacae and Klebsiella sp. The MIC of Glycyrrhiza glabra roots were 3μg/ml and 1μg/ml for E. coli and P. aeruginosa, respectively. The phytochemical analysis revealed the presence of tannins in leaf and root extracts and the concentration was found to be 3.161 and 2.455mg/ml, respectively at 530nm. This could be the possible explanation for the inhibitory effect of the extracts against the test organisms. 

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甘草根、叶提取物抑菌活性评价

甘草是一种传统的草本植物，生长在世界各地。它是一种非常甜，滋润，舒缓的草药，可以排毒和保护肝脏。它还具有强大的抗炎作用，在关节炎和口腔溃疡中也有应用。本研究采用琼脂孔/圆盘扩散法测定了甘草根和叶提取物对大肠杆菌、铜绿假单胞菌、阴沟肠杆菌和克雷伯氏菌的抑菌活性。冷浸法和索氏法提取液对试验菌均无抑制作用。用回流提取法制备的叶提取物和根提取物对大肠杆菌和铜绿假单胞菌均有较强的抗菌活性。甘草根对大肠杆菌和铜绿假单胞菌的MIC分别为3μg/ml和1μg/ml。植物化学分析表明，在530nm处，叶和根提取物中存在单宁，浓度分别为3.161和2.455mg/ml。这可能是对提取物对试验生物的抑制作用的可能解释。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of medical and bioengineering

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