ガスクロマトグラフ-燃焼-同位体比質量分析計によるホウレンソウ中のシュウ酸の炭素同位体組成分析法

Abstract

An analytical method to measure 13C abundance in 13C enriched oxalate using gas chromatograph/combustion/isotope ratio mass spectormeter (GC/C/IRMS) was established. The 13C atom% values of oxalate measured by GC/C/IRMS were nearly equal to those measured by automated nitrogen and carbon analyzer-mass spectrometer (ANCA-MS) giving a high degree of correlation (r2=0.9999) and good reproducibility (CV=0.224%). This method will be applicable in the study of oxalate metabolism in spinach.